Published: Dec. 21, 2022

Using the nematode C. elegans germline as a model system, we previously reported that PUF-8 (a PUF RNA-binding protein) and LIP-1 dual-specificity phosphatase) repress sperm fate at 20°C dedifferentiation of spermatocytes into mitotic cells (termed "spermatocyte dedifferentiation") 25°C. Thus, double mutants lacking both produce excess 20°C, their return to mitotically dividing via 25°C, resulting in tumors. To gain insight molecular competence for spermatocyte dedifferentiation, compared phenotypes three mutant strains – fem-3(q20gf), puf-8(q725; puf-8(q725); lip-1(zh15). Both fem-3(q20gf) produced like lip-1(zh15) mutants. Our results show was not observed mutants, but it more aggressive than These suggest MPK-1 (the ERK1/2 MAPK ortholog) activation by removing function absence promotes dedifferentiation. This idea confirmed using Resveratrol (RSV), potential activator human cells. Notably, significantly enhanced RSV treatment, its effect blocked mpk-1 RNAi. We, therefore, conclude are normally required inhibit tumorigenesis. Since these regulators broadly conserved, similar regulatory circuitry may control cellular tumorigenesis other organisms, including humans.

Language: Английский