Development of a Novel Anti-CD44 variant 6 Monoclonal Antibody for Multiple Applications against Colorectal Carcinomas DOI Open Access

Ryo Ejima,

Hiroyuki Suzuki, Tomohiro Tanaka

et al.

Published: Jan. 9, 2023

CD44 is a cell surface glycoprotein, and its isoforms are produced by the alternative splicing with standard variant exons. The exon containing (CD44v) overexpressed in carcinomas. CD44v6 one of CD44v, overexpression predicts poor prognosis colorectal cancer (CRC) patients. plays critical roles CRC adhesion, proliferation, stemness, invasiveness, chemoresistance. Therefore, promising target for diagnosis therapy CRC. In this study, we established anti-CD44 monoclonal antibodies (mAbs) immunizing mice CD44v3-10-overexpressed Chinese hamster ovary-K1 (CHO) cells. We then characterized them using enzyme-linked immunosorbent assay, flow cytometry, western blotting, immunohistochemistry. One clones (C44Mab-9; IgG1, kappa) reacted peptide 6-encoded region, indicating that C44Mab-9 recognizes CD44v6. Furthermore, CHO/CD44v3-10 cells or lines (COLO201 COLO205) cytometry. apparent KD CHO/CD44v3-10, COLO201, COLO205 was 8.1 × 10−9 M, 1.7 10−8 2.3 respectively. detected CD44v3-10 partially stained formalin-fixed paraffin-embedded tissues Collectively, useful detecting various applications.

Language: Английский

Critical role of keratinocytes in cutaneous immune responses DOI Creative Commons
Rakesh Kumar Gupta, Poonam Wasnik, Deepankar Mondal

et al.

Exploration of Immunology, Journal Year: 2024, Volume and Issue: 4(4), P. 502 - 522

Published: Aug. 27, 2024

Keratinocytes play an integral role in the human epidermis, serving as a barrier between internal and external environment. They are immune-competent cells involved both innate adaptive cutaneous immune responses, crucial for maintaining skin integrity. essential epidermal repair, facilitating proliferation re-epithelialization following injury. secrete pro-inflammatory markers such cytokines chemokines, which promote recruitment of inflammatory like polymorphs macrophages to site The response mediated by keratinocytes involves signaling molecules tumor necrosis factor (TNF), interleukin (IL)-1β, IL-6. Langerhans respond factors secreted keratinocytes, migrating towards draining lymph nodes activate T initiate response. Additionally, express Toll-like receptors (TLRs), enabling them detect molecular patterns pathogens. Recent studies have focused on understanding these interactions develop therapeutic strategies managing various diseases. Genetic defects underlie conditions psoriasis. We also discuss effect neuro-endocrinal interventions, associated corticosteroidogenic pathways, UV radiations maintain state homeostasis. This article underlines improves our function is developing more effective therapies against

Language: Английский

Citations

1
Development of a Novel Anti-CD44 variant 6 Monoclonal Antibody for Multiple Applications against Colorectal Carcinomas DOI Open Access

Ryo Ejima,

Hiroyuki Suzuki, Tomohiro Tanaka

et al.

Published: Jan. 9, 2023

CD44 is a cell surface glycoprotein, and its isoforms are produced by the alternative splicing with standard variant exons. The exon containing (CD44v) overexpressed in carcinomas. CD44v6 one of CD44v, overexpression predicts poor prognosis colorectal cancer (CRC) patients. plays critical roles CRC adhesion, proliferation, stemness, invasiveness, chemoresistance. Therefore, promising target for diagnosis therapy CRC. In this study, we established anti-CD44 monoclonal antibodies (mAbs) immunizing mice CD44v3-10-overexpressed Chinese hamster ovary-K1 (CHO) cells. We then characterized them using enzyme-linked immunosorbent assay, flow cytometry, western blotting, immunohistochemistry. One clones (C44Mab-9; IgG1, kappa) reacted peptide 6-encoded region, indicating that C44Mab-9 recognizes CD44v6. Furthermore, CHO/CD44v3-10 cells or lines (COLO201 COLO205) cytometry. apparent KD CHO/CD44v3-10, COLO201, COLO205 was 8.1 × 10−9 M, 1.7 10−8 2.3 respectively. detected CD44v3-10 partially stained formalin-fixed paraffin-embedded tissues Collectively, useful detecting various applications.

Language: Английский

Citations

2