Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (<em>Triticum aestivum</em>) DOI Open Access

Shefali Mishra,

Pradeep Sharma, Singh Rajender

et al.

Published: May 29, 2023

RNA interference is a highly conserved process in which non-coding small RNAs (sRNA) modulate gene expression at the post-transcriptional level influencing plant growth and development. Dicer-like (DCL), Argonaute (AGO) RNA-dependent polymerase (RDR) are core elements involved silencing their families have been explored many plants. However, these genes response to abiotic stresses not yet well wheat. In this study, 82 AGO, 31 DCL, RDR were identified phylogenetic analysis of proteins showed that clustered into ten, four clades respectively. RNA-seq revealed constitutive AGO1, AGO9, DCL2 family tissues under normal stress conditions, whereas RDR1 known engage siRNA biogenesis higher levels wheat leaf tissues. Our findings build foundation for comparative genomics analyses cereal crops, as new insights functional complexity responses, critical understanding processes underlying responses.

Language: Английский

Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (<em>Triticum aestivum</em>) DOI Open Access

Shefali Mishra,

Pradeep Sharma, Singh Rajender

et al.

Published: May 29, 2023

RNA interference is a highly conserved process in which non-coding small RNAs (sRNA) modulate gene expression at the post-transcriptional level influencing plant growth and development. Dicer-like (DCL), Argonaute (AGO) RNA-dependent polymerase (RDR) are core elements involved silencing their families have been explored many plants. However, these genes response to abiotic stresses not yet well wheat. In this study, 82 AGO, 31 DCL, RDR were identified phylogenetic analysis of proteins showed that clustered into ten, four clades respectively. RNA-seq revealed constitutive AGO1, AGO9, DCL2 family tissues under normal stress conditions, whereas RDR1 known engage siRNA biogenesis higher levels wheat leaf tissues. Our findings build foundation for comparative genomics analyses cereal crops, as new insights functional complexity responses, critical understanding processes underlying responses.

Language: Английский

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