Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams after Thawing

Published: May 30, 2023

Glutathione (GSH) and melatonin (MLT) are two antioxidants that have been described as having a protective role against oxidative stress in several animal species, improving sperm quality survival after cryopreservation. Since there is no knowledge on the simultaneous use of these substances concerning its effects ram semen cryopreservation, this study aimed to evaluate MLT, GSH, or their association, vitro parameters thawing. During eight weekly sessions, from three Merino rams was pooled, divided into four aliquots, diluted with an egg yolk-based extender without (control) (MLT 1mM, GSH 5mM, MLT 1mM + 5mM). Diluted evaluated before freezing process. Supplementation fresh at 5 mM had deleterious effect total motility progressive (TPM), curvilinear velocity (VCL), straight-line (VSL), average-path (VAP), linearity (LIN), straightness (STR) increased slow spermatozoon (%). 1 also has negative TPM, VSL, STR semen. In thawed semen, TPM regarding group control. Also, combination both attenuates 5mM VSL BCF. These results indicate impairs does not improve kinetic suggest when combined plays effects.

Language: Английский

Effects of Adding Glutathione to AndroMed Diluent on Intact Plasma and Acrosome Membranes, and Progressive Motility of Cattle Spermatozoa During Freezing Processes

World s Veterinary Journal, Journal Year: 2023, Volume and Issue: 13(4), P. 561 - 570

Published: Dec. 25, 2023

Adding endogenous antioxidants to the diluent is significantly associated with semen quality during freezing process. This study aimed investigate effects of adding glutathione AndroMed on preservation crucial sperm attributes, namely, intact plasma membrane (IPM), acrosome (IAM), and progressive motility Bali cattle spermatozoa. A completely randomized design was used, spermatozoa samples were obtained from a divided into two treatment groups (36 in each group six replications), namely pure as control addition (1 mmol) AndroMed. Each replicated times evaluated at three stages, including post-dilution, post-equilibration, post-thawing, for properties. The results indicated that fresh had motility, IAM, IPM 75%, 89%, 88%, respectively. During process, there significant decrease quality, after dilution post-equilibration post-thawing both groups. Meanwhile, 1 mmol difference increasing stage freezing, post thawing when compared controls. Based results, it can be concluded enhanced integrity semen,

Language: Английский