Isolation of a Novel Pythium Species, P. thermoculicivorax, and Trichoderma sp. from Natural Enzootic Mosquito Larval Infections DOI Creative Commons
Ross Joseph,

Constance Darrisaw,

Aaron Lloyd

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(3), P. 199 - 199

Published: March 5, 2024

Only a handful of microbial mosquito larval pathogens have been described to date. Sampling several natural enzootic infections larvae in southwestern Florida indicated the presence capable extensive mortality. A microscopic analysis one sample site revealed apparent growth Pythium-like microbe on larvae, with highest degree infection observed siphon and head regions. Structures consistent sporangia were seen infected insects after lactophenol blue staining, higher-resolution scanning electron microscopy (SEM) micrographs showed encysted zoospores targeting The isolate was single-colony purified, molecular identification ITS COX1 loci coupled phylogenetic reconstruction that belonged Pythium genus but distinct from its closest characterized species, P. inflatum. Morphological features characterized, showing rapid all mycological media tested relatively high thermotolerance, robust at 37 °C; hence, it designated thermoculicivorax. second series resulted three Trichoderma isolates, similarity T. strigosum other two clustering closely asperellum. These data highlight occurrence potentially as resource for new pathogens.

Language: Английский

Aedes (Ochlerotatus) scapularis, Aedes japonicus japonicus, and Aedes (Fredwardsius) vittatus (Diptera: Culicidae): Three Neglected Mosquitoes with Potential Global Health Risks DOI Creative Commons

Vivian Petersen,

Micael Santana,

Maria Karina-Costa

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(8), P. 600 - 600

Published: Aug. 8, 2024

More than 3550 species of mosquitoes are known worldwide, and only a fraction is involved in the transmission arboviruses. Mosquitoes sylvatic semi-sylvatic habitats may rapidly adapt to urban parks metropolitan environments, increasing human contact. Many these have been found naturally infected with arboviruses from Alphaviridae, Flaviviridae, Bunyaviridae families, many being cause medically important diseases. However, there gap knowledge about vector status newly invasive their potential threat domestic animal populations. Due rapid distribution, adaptation anthropophilic habits, some neglected mosquito deserve more attention regarding role as secondary vectors. Taking factors into account, we focus here on Aedes (Ochlerotatus) scapularis (Rondani), japonicus (Theobald), (Fredwardsius) vittatus (Bigot) that become disease We further discuss importance how such urbanization, climate change, globalization profoundly alter dynamics increase participation propagating

Language: Английский

Citations

4
Bacillus velezensis as a Novel Species for Mosquito Control: Impacts of Exposure on Key Life History Traits of Culex quinquefasciatus DOI Creative Commons
Abdullah A. Alomar

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 434 - 434

Published: April 20, 2025

Bacillus velezensis is a promising mosquito vector biocontrol agent due to its potent larvicidal properties. This study investigated the lethal and sublethal effects of exposure crude toxin B. strain WHk23 on Culex quinquefasciatus. Toxicity bioassays showed median concentration 28.30 µg/mL for third instar larvae. In addition direct larval mortality, exposures resulted in concentration-dependent decreases survival, fecundity, fertility. Exposure different concentrations was found accelerate development, resulting larger females, yet those females exhibited lower lifespan diminished reproductive success. These results suggest that not only reduces populations but also has lasting important life history parameters, ultimately lowering vectorial capacity disease transmission risk. provides valuable insights into consequences exposure. Given properties, represents vectors.

Language: Английский

Citations

0
Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction of Vector Competence DOI Open Access
Ricardo de Melo Katak, Amanda Montezano Cintra, Bianca Burini Kojin

et al.

Published: June 23, 2023

Mosquitoes transmit pathogens that cause human diseases such as malaria, Dengue fever, Chikungunya, yellow Zika and filariasis. Chemical pesticides are effective instruments for reducing disease transmission managing mosquito populations. However, these chemicals have drawbacks high production costs negative effects on the environment non-target organisms. Furthermore, mosquitoes develop resistance to chemical insecticides, they become less effective. As a result, researchers investigating novel insecticides. Advances in microbial biotechnology resulted wide range of value-added products, including biopesticides agricultural public health purposes. In this review, we highlight mainly untapped biotechnological potential microorganisms vector control reduction. Also, underline importance more basic research improved translational methodologies, encouraging efforts bridge gap between academic interventions.

Language: Английский

Citations

4
Isolation of a Novel Pythium Species, P. thermoculicivorax, and Trichoderma sp. from Natural Enzootic Mosquito Larval Infections DOI Creative Commons
Ross Joseph,

Constance Darrisaw,

Aaron Lloyd

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(3), P. 199 - 199

Published: March 5, 2024

Only a handful of microbial mosquito larval pathogens have been described to date. Sampling several natural enzootic infections larvae in southwestern Florida indicated the presence capable extensive mortality. A microscopic analysis one sample site revealed apparent growth Pythium-like microbe on larvae, with highest degree infection observed siphon and head regions. Structures consistent sporangia were seen infected insects after lactophenol blue staining, higher-resolution scanning electron microscopy (SEM) micrographs showed encysted zoospores targeting The isolate was single-colony purified, molecular identification ITS COX1 loci coupled phylogenetic reconstruction that belonged Pythium genus but distinct from its closest characterized species, P. inflatum. Morphological features characterized, showing rapid all mycological media tested relatively high thermotolerance, robust at 37 °C; hence, it designated thermoculicivorax. second series resulted three Trichoderma isolates, similarity T. strigosum other two clustering closely asperellum. These data highlight occurrence potentially as resource for new pathogens.

Language: Английский

Citations

1