Elevated UTI Biomarkers in Symptomatic Patients with Urine Microbial Densities of 10,000 CFU/mL Indicate a Lower Threshold for Diagnosing UTIs

Diagnostics, Journal Year: 2023, Volume and Issue: 13(16), P. 2688 - 2688

Published: Aug. 16, 2023

The literature lacks consensus on the minimum microbial density required for diagnosing urinary tract infections (UTIs). This study categorized densities of urine specimens from symptomatic UTI patients aged ≥ 60 years and correlated them with detected levels immune response biomarkers neutrophil gelatinase-associated lipocalin (NGAL), interleukin-8 (IL-8), interleukin-1-beta (IL-1β). objective was to identify associated significant elevation these in order determine an optimal threshold UTIs. Biobanked midstream voided samples were analyzed identification quantification using standard culture (SUC) multiplex-polymerase chain reaction (M-PCR) testing, while NGAL, IL-8, IL-1β measured via enzyme-linked immunosorbent assay (ELISA). all significantly elevated at 10,000 cells/mL when M-PCR (p < 0.0069) or equivalent colony-forming units (CFUs)/mL SUC 0.0104) compared no detectable microbes. With both PCR SUC, a two more well > CFU/mL, respectively. association between ≥10,000 cells CFU per mL suggests that this lower may be suitable than 100,000 CFU/mL

Language: Английский

Urine Biomarkers Individually and as a Consensus Model Show High Sensitivity and Specificity for Detecting UTIs

Published: July 3, 2023

We aimed to determine if infection-associated urine biomarkers can differentiate true urinary tract infection (UTI) from non-UTI controls. Midstream clean-catch samples were collected asymptomatic volunteers and symptomatic subjects &gt; 60 years old diagnosed with presumptive UTI in a specialty setting. Microbial identification density assessed using multiplex PCR/pooled antibiotic susceptibility test (M-PCR/P-AST) standard culture (SUC). Three (NGAL, IL-8, IL-1β) measured the same specimens. Definitive cases had positive microorganism detection by SUC M-PCR, while definitive regardless of microbial detection. observed strong correlation (R2 ≈ 1) between NGAL, IL-1β. Biomarker consensus criteria two or more sensitivity 90.2%, specificity 91.2%, predictive value (PPV) 91.7%, negative (NPV) 89.7%, accuracy 90.7%, likelihood ratio 10.28, 0.11 differentiating cases, density. IL-1β showed significant elevation microbe compared without identification. exhibited high distinguishing cases.

Language: Английский

UTI Biomarkers in Symptomatic Patients are Significantly Elevated at Microbial Densities of 10,000 cells or CFUs per mL Indicating a Lower Microbial Density Threshold for Diagnosing UTIs

Published: July 3, 2023

The literature lacks consensus on the minimum microbial density required for diagnosing urinary tract infections (UTIs). This study categorized densities of urine specimens from symptomatic presumptive UTI patients and correlated them with detected levels immune response biomarkers NGAL, IL-8, IL-1β. objective was to identify changes in at different determine an optimal threshold UTIs. Midstream clean-catch samples were analyzed identification quantification using SUC M-PCR, while IL-1β measured ELISA. all significantly higher subjects ≥ 10,000 cells/mL by M-PCR (p &lt; 0.0069) or equivalent CFUs/mL 0.0104) compared no detectable microbes. With both PCR SUC, a two more elevated well &gt; CFU/mL, respectively. association between cells CFU per mL suggests that this lower may be suitable These findings challenge widely used 100,000 mL, which overlook many active cases.

Language: Английский