Synergistic Antimicrobial and Antibiofilm Activity of Panax Ginseng, Symphytum Officinale, and Metronidazole against P. gingivalis DOI Open Access
Salah M. Ibrahim, Abbas S. Al-Mizraqchi, Julfikar Haider

et al.

Published: Aug. 21, 2023

Background: The biofilm-forming bacteria Porphyromonas gingivalis (P. gingivalis) is primarily responsible for periodontal disorders, which continue to be a global health issue. Despite the widespread use of antibiotics like metronidazole, other potent therapeutic crucial quorum sensing molecule implicated in formation P. biofilm. Approaches are now being explored due growing challenge antibiotic resistance. Objective: In particular, chemicals from Symphytum Officinale (S) and Panax Ginseng (G) plants will examined this study assess if they have ability inhibit biofilm prevent development acylated homoserine lactones (AHLs), molecule. Materials Methods: With Metronidazole serving as control, antibacterial effects these substances (Symphytum Ginseng) were against standard strain clinical isolate gingivalis. Dilutions plant extracts used either alone (G+S) or conjunction with metronidazole (G+S+F), their activity using susceptibility test (inhibition zone), inhibition disruption assay (optical density) (AHL). Results: combinations officinale, (S+G+F) showed maximum effectiveness highest zone (25.000±0.001 mm) (98.46%), comparable G+S 24.341±0.593 mm 97.76%), significantly different degree treatment scenarios. Additionally, plant's specific concentrations had significant effect on suppression generation AHL (p < 0.05). Conclusion: According preliminary research, plant-derived substances, especially when paired inhibited growth providing valuable information innovative approaches periodontitis biofilm-associated illnesses.

Language: Английский

Therapeutic Potential of Ginsenosides on Bone Metabolism: A Review of Osteoporosis, Periodontal Disease and Osteoarthritis DOI Open Access
S KO

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5828 - 5828

Published: May 27, 2024

Ginsenosides, bioactive compounds from the genus Panax, have potential therapeutic effects on diverse ailments, including diabetes. Emerging evidence suggests their involvement in bone metabolism. The present review summarizes current understanding of ginsenosides osteoporosis, periodontal disease, and osteoarthritis. Their mechanisms action include osteoblasts, osteoclasts, ligament fibroblasts (PDLFs), chondrocytes, which are pivotal maintaining bone, tissue, cartilage homeostasis. Ginsenosides may exert beneficial by enhancing PDLF osteoblast activity, suppressing osteoclast function, augmenting chondrocyte synthesis matrix, mitigating connective tissue degradation. Moreover, they possess antioxidant, anti-inflammatory, antimicrobial, anti-pyroptotic properties. efficacy increasing density, ameliorating periodontitis, alleviating osteoarthritis symptoms has been demonstrated preclinical studies using animal models. In terms mechanism action, modulate cellular differentiation, key signaling pathway molecules, such as mitogen-activated protein kinases (MAPKs), while also regulating various mediators. Furthermore, symptomatic relief observed models lends further credence to utility. However, translate these findings into clinical practice, rigorous investigations imperative ascertain safety, efficacy, optimal dosing regimens human subjects.

Language: Английский

Citations

4
Metronidazole Potentiation by Panax Ginseng and Symphidium Officinale: A New Strategy for P. gingivalis Infection Control DOI Open Access
Salah M. Ibrahim,

Abass sabri al-mizragchi,

Julfikar Haider

et al.

Published: July 10, 2023

Background: The important periodontal disease pathogen Porphyromonas gingivalis produces large biofilms that increase its pathogenicity. Finding natural antimicrobial agents is crucial because of the rise in antibiotic resistance. Objective: purpose this study was to determine if plant extracts such as Symphytum officinale (S) and Panax ginseng (G) were effective against P. separately combination with a common Metronidazole (F). Materials Procedures: Six different dilutions produced G F S using two-fold serial dilution technique. To evaluate effects these substances biofilm inhibition experiments conducted. Plaque samples collected from periodontitis patients isolate standard strain (ATCC 33277) bought. Additionally, Acylated Homoserine Lactones (AHLs) detection carried out look for any activity would interfere quorum sensing. GraphPad prism used statistical analysis p value < 0.05. Results: combinations Officinale (S+F) showed maximum effectiveness (98.7%), which better than G+F (98.2%) substantial variations levels treatment regimes. Notably, patient more active strain. their at particular had notable inhibitory on generation AHL (p <0.05). Conclusion: highlights possibility Ginseng treatments AHLs, both own Metronidazole. These organic may open door cutting-edge methods treating disorders.

Language: Английский

Citations

4
Preparation and Characterization of Ginseng Gel and In Vivo Evaluation of Its Clinical Efficacy in Generalized Chronic Gingivitis Patients DOI Open Access

Johnisha Harris,

Arvina Rajasekar

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: March 27, 2024

Aim The aim of the present study was to prepare and characterize ginseng gel then evaluate its clinical efficacy in terms plaque index (PI), gingival (GI) among generalized chronic gingivitis patients. Materials methods Ginseng prepared using 250 g powder. antimicrobial activity chlorhexidine checked at various concentrations (25, 50, 75, 100, 125, 150, 175, 200, 225, 275 µg) against anaerobic organisms find concentration with maximum activity. highest subjected vivo analysis. A total 30 patients were scaling divided into two groups for intraoral application - Group I (ginseng gel) II (chlorhexidine one month. parameters PI, GI measured baseline (pre scaling) month (post comparing (Hexigel gluconate 1.0% w/w). Independent t test paired done statistical Results At µg, showed antibacterial action oral microorganisms. In I, reduction PI from (2.52±0.02) follow up after (0.75±0.05), (2.2±0.35) (0.9±0.02). II, (2.54±0.01) (0.79±0.02), (2.1±0.42) (0.8±0.01). Conclusion equal GI. Though effective lower concentrations, it has considerable adverse effects such as taste alteration. Hence is better encourage use herbal-based products management prevent side synthetic preparations.

Language: Английский

Citations

0
Synergistic Antimicrobial and Antibiofilm Activity of Panax Ginseng, Symphytum Officinale, and Metronidazole against P. gingivalis DOI Open Access
Salah M. Ibrahim, Abbas S. Al-Mizraqchi, Julfikar Haider

et al.

Published: Aug. 21, 2023

Background: The biofilm-forming bacteria Porphyromonas gingivalis (P. gingivalis) is primarily responsible for periodontal disorders, which continue to be a global health issue. Despite the widespread use of antibiotics like metronidazole, other potent therapeutic crucial quorum sensing molecule implicated in formation P. biofilm. Approaches are now being explored due growing challenge antibiotic resistance. Objective: In particular, chemicals from Symphytum Officinale (S) and Panax Ginseng (G) plants will examined this study assess if they have ability inhibit biofilm prevent development acylated homoserine lactones (AHLs), molecule. Materials Methods: With Metronidazole serving as control, antibacterial effects these substances (Symphytum Ginseng) were against standard strain clinical isolate gingivalis. Dilutions plant extracts used either alone (G+S) or conjunction with metronidazole (G+S+F), their activity using susceptibility test (inhibition zone), inhibition disruption assay (optical density) (AHL). Results: combinations officinale, (S+G+F) showed maximum effectiveness highest zone (25.000±0.001 mm) (98.46%), comparable G+S 24.341±0.593 mm 97.76%), significantly different degree treatment scenarios. Additionally, plant's specific concentrations had significant effect on suppression generation AHL (p < 0.05). Conclusion: According preliminary research, plant-derived substances, especially when paired inhibited growth providing valuable information innovative approaches periodontitis biofilm-associated illnesses.

Language: Английский

Citations

1