From Entry to Outbreak in a High School Setting: Clinical and Wastewater Surveillance of a Rare SARS-CoV-2 Variant

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 477 - 477

Published: March 27, 2025

In December 2021, an outbreak of the SARS-CoV-2 B.1.640.2 variant, potentially originating from Cameroon, was investigated among schoolchildren in Germany. The index case, adult who had recently returned a three-week stay Republic Congo, introduced variant into school setting via their children, resulting subsequent transmission within and ultimately to hospital ward. Whole-genome sequencing viral samples identified both B.1.640.1 lineages. This highlights unpredictable nature emerging variants emphasizes importance early detection containment mitigate high-risk populations. Notably, wastewater surveillance detected during study peri-od, reinforcing utility wastewater-based epidemiology as complementary tool for warning novel variants. These findings underline critical need timely research adherence quarantine measures enhance control efforts.

Language: Английский

The Rise and Fall of Omicron BA.1 Variant as Seen in Wastewater Supports Epidemiological Model Predictions

Published: July 20, 2023

The COVID-19 pandemic caused by the SARS-CoV-2 virus has inflicted significant mortality and morbidity worldwide. Continuous mutations have led to emergence of new variants. Omicron BA.1 sub-lineage prevailed as dominant variant globally at beginning 2022 but was subsequently replaced BA.2 in numerous countries. Wastewater-based epidemiology (WBE) offers an efficient tool for capturing viral shedding from infected individuals, enabling early detection potential outbreaks without relying solely on community cooperation clinical testing resources. This study integrated RT-qPCR assays detecting general its variants levels wastewater into a modified triple susceptible-infected-recovered-susceptible (SIRS) model. Omicron-BA.1 observed, replacing presence predecessor, Delta variant. Comparative analysis between data SIRS model effectively described subsequent waves, with decline aligning diminished below threshold wastewater. demonstrates WBE valuable future pandemics. Furthermore, analyzing sensitivity different parameters, we are able deduce real-life values cross-variant immunity probabilities, emphasizing asymmetry their strength.

Language: Английский

Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8839 - 8839

Published: Aug. 14, 2024

Wastewater-based surveillance has emerged as an important method for monitoring the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). This study investigated presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time Polymerase Chain Reaction (PCR). Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive SARS-CoV-2. these, 13 sequences sufficient length determine lineages obtained phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Language: Английский