The Effect of a TLR3 Agonist on Airway Allergic Inflammation and Viral Infection in Immunoproteasome-Deficient Mice DOI Creative Commons
Niccolette Schaunaman,

Taylor Nichols,

Diana Cervantes

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(9), P. 1384 - 1384

Published: Aug. 29, 2024

Allergic asthma is characterized by increased type 2 inflammation, including eosinophils. Subjects with allergic have recurrent symptoms due to their constant exposure environmental allergens, such as house dust mite (HDM), which can be further exacerbated respiratory infections like rhinovirus. The immunoproteasome (IP) a proteolytic machinery that induced inflammatory mediators during virus infection, but the role of IP in airway inflammation rhinovirus infection remains unknown. Wild-type (WT) and knockout (KO) mice were challenged HDM. At 48 h after last HDM challenge, infected 1B (RV-A1B) for 24 h. After RV-A1B treatment, KO (vs. WT) had significantly more lung eosinophils neutrophils, well higher viral load, less IFN-beta expression, compared WT mice. A TLR3 agonist polyinosinic-polycytidylic acid (Poly I:C) treatment HDM-challenged expression reduced minimal effect on number cells. Our data suggest an important mechanism functioning prevent excessive allergen-exposed mice, Poly I:C could therapeutically effective enhancing antiviral response lessening burden lungs deficiency.

Language: Английский

Evaluation of Adipokine Status and Leptin Receptor Gene Polymorphism in Patients with Severe Asthma DOI Creative Commons

Saule Maimysheva,

L. K. Karazhanova, A. Yu. Orekhov

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(9), P. 1154 - 1154

Published: May 1, 2025

Background: Severe and difficult-to-control asthma occurs in 3-10% of patients developed countries. The aim our study was to investigate the association prognostic role leptin adiponectin, as well receptor gene polymorphism Gln223Arg, with severe asthma. Methods: present included 200 hospitalized Department Pulmonology between January 2018 December 2021. In all patients, addition routine clinical investigations, their ratio were analyzed, levels pro-inflammatory cytokines (IL-6, IL-8 TNF-alpha). External respiratory function also assessed. LEPR Gln223Arg single-nucleotide polymorphisms genotyped by real-time PCR method. Results: Patients randomized into two groups, depending on severity asthma: an uncontrolled group a controlled group, according GINA criteria. Among asthma, 101 subjects (74.3%) had metabolic syndrome (p < 0.001). There inverse adiponectin/leptin eosinophil count (B = -0.305, p 0.001), IL-6 -0.026, -0.062, 0.001) TNF-alpha -0.047, direct correlation level FEV1 0.121, FVC 0.104, A probable homozygous A/A allele increased risk shown 0.007). Conclusions: Leptin genotype may be associated higher probability developing

Language: Английский

Citations

0

Asthma Phenotypes in the Era of Personalized Medicine DOI Open Access
Víctor González-Uribe,

Sergio Jesús Romero-Tapia,

José A. Castro-Rodrı́guez

et al.

Published: July 24, 2023

Asthma is a prevalent disease that around 300 million worldwide, resulting in substantial morbidity, mortality, and economic burden on global scale. New clinical laboratory research has shed light the immunology causing asthma. now recognized as heterogeneous disease. A personalized medicine based classification of asthma by endotype linking observable characteristics to immunological mechanisms. Identifying mechanisms essential for better characterizing patients personalizing therapeutic approaches with novel biological agents target specific immune pathways. This article provides summary major involved pathogenesis emergence disease&#039;s phenotypic features, well individualized treatment severe subtypes

Language: Английский

Citations

4

Breath and Sputum Analyses in Asthmatic Patients: An Overview DOI Creative Commons
Piera Soccio, Carla Maria Irene Quarato, Pasquale Tondo

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(16), P. 1355 - 1355

Published: Aug. 14, 2024

Recent advancements in asthma management include non-invasive methodologies such as sputum analysis, exhaled breath condensate (EBC), and fractional nitric oxide (FeNO). These techniques offer a means to assess airway inflammation, critical feature of asthma, without invasive procedures. Sputum analysis provides detailed insights into inflammation patterns cellular composition, guiding personalized treatment strategies. EBC collection, reflecting bronchoalveolar lining fluid window physiology. FeNO emerges pivotal biomarker, offering eosinophilic aiding diagnosis, monitoring, the prediction exacerbation risks. Despite inherent limitations, each method offers valuable tools for more comprehensive assessment asthma. Combining these with traditional methods like spirometry may lead plans improved patient outcomes. Future research is crucial refine protocols, validate biomarkers, establish guidelines order enhance tailored therapeutic strategies

Language: Английский

Citations

1

The Effect of a TLR3 Agonist on Airway Allergic Inflammation and Viral Infection in Immunoproteasome-Deficient Mice DOI Creative Commons
Niccolette Schaunaman,

Taylor Nichols,

Diana Cervantes

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(9), P. 1384 - 1384

Published: Aug. 29, 2024

Allergic asthma is characterized by increased type 2 inflammation, including eosinophils. Subjects with allergic have recurrent symptoms due to their constant exposure environmental allergens, such as house dust mite (HDM), which can be further exacerbated respiratory infections like rhinovirus. The immunoproteasome (IP) a proteolytic machinery that induced inflammatory mediators during virus infection, but the role of IP in airway inflammation rhinovirus infection remains unknown. Wild-type (WT) and knockout (KO) mice were challenged HDM. At 48 h after last HDM challenge, infected 1B (RV-A1B) for 24 h. After RV-A1B treatment, KO (vs. WT) had significantly more lung eosinophils neutrophils, well higher viral load, less IFN-beta expression, compared WT mice. A TLR3 agonist polyinosinic-polycytidylic acid (Poly I:C) treatment HDM-challenged expression reduced minimal effect on number cells. Our data suggest an important mechanism functioning prevent excessive allergen-exposed mice, Poly I:C could therapeutically effective enhancing antiviral response lessening burden lungs deficiency.

Language: Английский

Citations

1