Microbially-Mediated Phenolic Catabolites Exert Differential Geno-Protective Activities in Normal and Adenocarcinoma Cell Lines DOI Open Access
Brian Murphy, Cheryl Latimer, Sara Dobani

et al.

Published: Aug. 9, 2023

Ageing is associated with a decline in both Nrf2 activity and DNA repair efficiency, leading to the accumulation of damage an increased risk cancer. Understanding mechanisms behind level damaged during ageing crucial for developing interventions mitigate age-related cancer improve overall health older individuals. (Poly)phenols are plant-derived bioactive compounds that undergo metabolism into simpler phenolic catabolites following ingestion. While these have been various benefits, further analysis their bioavailable necessary. To this end, four derived from colonic microbiota were investigated ability reduce response oxidative challenge modulate Nrf2-Antixoidant Response Element pathway. Normal adenocarcinoma colonocyte cell lines (CCD 841 CoN, & HT29 respectively) exposed sub cytotoxic concentrations (>10 µM,) hydroxylated (4-hydroxybenzoic acid, 3-(3’-hydroxyphenyl) propionic acid) non-hydroxylated (benzoic acid or 3-phenylpropionic acids. Using COMET assay, each catabolite demonstrated significant (p<0.001) geno-protective activity, albeit varying potency. They also significantly modulated expression key genes Nrf2-ARE pathway, including Nrf2, Heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase quinone 1 (NQO1). Hydroxylation acids appeared limited effect respect bioactivity. Overall, colon metabolites, when assessed at physiologically relevant concentrations, reduced normal cells challenge, mediated – least part via upregulation

Language: Английский

Microbially-Mediated Phenolic Catabolites Exert Differential Geno-Protective Activities in Normal and Adenocarcinoma Cell Lines DOI Open Access
Brian Murphy, Cheryl Latimer, Sara Dobani

et al.

Published: Aug. 9, 2023

Ageing is associated with a decline in both Nrf2 activity and DNA repair efficiency, leading to the accumulation of damage an increased risk cancer. Understanding mechanisms behind level damaged during ageing crucial for developing interventions mitigate age-related cancer improve overall health older individuals. (Poly)phenols are plant-derived bioactive compounds that undergo metabolism into simpler phenolic catabolites following ingestion. While these have been various benefits, further analysis their bioavailable necessary. To this end, four derived from colonic microbiota were investigated ability reduce response oxidative challenge modulate Nrf2-Antixoidant Response Element pathway. Normal adenocarcinoma colonocyte cell lines (CCD 841 CoN, & HT29 respectively) exposed sub cytotoxic concentrations (>10 µM,) hydroxylated (4-hydroxybenzoic acid, 3-(3’-hydroxyphenyl) propionic acid) non-hydroxylated (benzoic acid or 3-phenylpropionic acids. Using COMET assay, each catabolite demonstrated significant (p<0.001) geno-protective activity, albeit varying potency. They also significantly modulated expression key genes Nrf2-ARE pathway, including Nrf2, Heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase quinone 1 (NQO1). Hydroxylation acids appeared limited effect respect bioactivity. Overall, colon metabolites, when assessed at physiologically relevant concentrations, reduced normal cells challenge, mediated – least part via upregulation

Language: Английский

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