The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol DOI Creative Commons

Giulio Bevilacqua,

Roberta Corvino,

Anna Laura Capriotti

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1024 - 1024

Published: Dec. 7, 2024

The National Cancer Institute (NCI) recognizes the potential of technologies based on use nanoparticles (NPs) in revolutionizing clinical approaches to diagnosis and prognosis cancer. Recent research suggests that once NPs come into contact with biological fluid cancer patients, they are covered by proteins, forming a “protein corona” composed hundreds plasma proteins. concept personalized, disease-specific protein corona, demonstrating substantial differences NP corona profiles between patients without cancer, has been introduced. We developed design an experimental prospective single-center study allocated 1:1:1 ratio one three arms: untreated benign prostatic hyperplasia (BPH), non-metastatic prostate (PCa), metastatic starting systemic therapies new androgen-targeted agents or taxanes. protocol aims develop implement sensitive nanotools two distinct objectives: First, capable selectively binding detecting biomarkers order build predictive diagnostic model effectively discriminate patient sera affected BPH PCa. Secondly, within population PCa, case initial advanced diagnosis, objective is find predicting response treatments improve precision efficiency monitoring treatment outcomes. For metabolite experiments, we cross-reactive sensor array platform detection capacity made liposomal formulations different surface charges. proteomic-NP studies, proteins were identified quantified using nano-high-performance LC (nanoHPLC) coupled MS/MS (nanoHPLC−MS/MS). Metabolites instead analyzed untargeted metabolomic approach. Compared previous review articles, novelty this represented analysis possible applications focused PCa presentation phase

Language: Английский

Tissue Engineering and Biosensing Applications of Carbon-Based Nanomaterials DOI Creative Commons
Seydanur Yücer, Begüm Sarac, Fatih Çiftçi

et al.

Biomedical Engineering Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100145 - 100145

Published: Jan. 1, 2025

Language: Английский

Citations

2
Revolution in Cancer Treatment: How Are Intelligently Designed Nanostructures Changing the Game? DOI Open Access

Désirée Gül,

Burcu Önal Acet, Qiang Lü

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5171 - 5171

Published: May 9, 2024

Nanoparticles (NPs) are extremely important tools to overcome the limitations imposed by therapeutic agents and effectively biological barriers. Smart designed/tuned nanostructures can be effective for cancer treatment. The selection design of adjustment size surface properties important, especially some precision treatments drug delivery (DD). By designing specific methods, an era opened in biomedical field personalized precise Here, we focus on advances nanostructures, as well how structure shape, size, charge, fluids (BFs) affected. We discussed applications specialized therapy head neck (HNC), which is a difficult aggressive type treat, give impetus novel treatment approaches this field. also comprehensively touched shortcomings, current trends, future perspectives when using cancer.

Language: Английский

Citations

6
Application of Nanoparticles in Cancer Treatment: A Concise Review DOI Open Access

Mariana Sell,

Ana Rita Lopes,

Maria Escudeiro

et al.

Published: Oct. 10, 2023

Cancer is among the leading causes of death worldwide. Therefore, timely diagnosis and appropriate treatment are very important. There many disadvantages that come with traditional cancer treatments, such as chemotherapy radiotherapy. In these specific drug concentration affects not only tumor site but also healthy tissues or organs. One foremost promising uses nanotechnology in field medical technology targeting can be achieved due to manipulation materials at a nanometric scale. Nanotechnology offers benefits terms therapy by enhancing it reducing its adverse effects guiding drugs selectively target cells. addition, use minute amounts medicine lead cost savings. Furthermore, nanoparticles used imaging agents improve diagnostics, therapeutics, management. Thus, this review has focused on different types therapy, their action mechanisms, applications diagnosis, imaging, treatment. This sums up parameters need considered when designing systems for while considering desired characteristics from biological point view.

Language: Английский

Citations

12
Green Myco-Synthesis of Zinc Oxide Nanoparticles Using Cortinarius sp.: Hepatoprotective, Antimicrobial, and Antioxidant Potential for Biomedical Applications DOI Creative Commons

Uzma Fazal,

Ahmad Zada, Muhammad Hanif

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 956 - 956

Published: April 22, 2025

The transformative effect of nanotechnology is revolutionizing medicine by introducing new therapeutic approaches. This study explores the utilization aqueous extract from mushroom (Cortinarius sp.) used as a reducing agent to prepare zinc oxide myco-nanoparticles (ZnO-MNPs) in an eco-friendly manner. synthesis ZnO-MNPs has been confirmed various characterization studies, including UV-vis spectroscopy, which revealed absorption peak at 378 nm; X-ray diffraction (XRD) analysis, wurtzite hexagonal structure; and Fourier transform infrared spectra (FTIR), showed stabilizing agents around ZnO-MNPs. effectiveness anti-cancer was evaluated monitoring liver biochemical parameters against hepatotoxicity caused carbon tetrachloride (CCl4) Balb C mice. results that levels catalase, glutathione (GSH), total protein were significantly lower, while alanine aminotransferase (ALT), aspartate (ASAT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), melanin dialdehyde (MDA), bilirubin (TB) higher each CCl4 treatment groups. ZnO-MNP reduced toxicological effects but did not completely restore accumulation. antimicrobial efficacy investigated potential common pathogens, Bacillus subtilis (29.05 ± 0.76), meurellus (27.05 0.5), Acetobacter rhizospherensis (23.36 Escherichia coli (25.86 0.80), antifungal activity relatively lower. Moreover, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay are strong antioxidant agents. Overall, these findings highlight myco-synthesized ZnO-NPs combating pathogenic diseases, their promising role cancer therapy, biomaterial option for future applications.

Language: Английский

Citations

0
Anticancer Nanoparticle Carriers of the Proapoptotic Protein Cytochrome c DOI Creative Commons
Alexandar M. Zhivkov, S. Hristova, Trifon T. Popov

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 305 - 305

Published: Feb. 26, 2025

This review discusses the literature data on synthesis, physicochemical properties, and cytotoxicity of composite nanoparticles bearing mitochondrial protein cytochrome c (cytC), which can act as a proapoptotic mediator in addition to its main function an electron carrier transport chain. The introduction exogenous cytC via absorption particles, phagocytosis colloid particles submicrometric size, or receptor-mediated endocytosis cancer cells, initiates process apoptosis—a multistage cascade biochemical reactions leading complete destruction cells. CytC–carrier have potential for use treatment neoplasms with superficial localization: skin, mouth, stomach, colon, etc. approach solve two problems anticancer therapy: selectivity non-toxicity. Selectivity is based incapability normal cell absorb (nano)particles, except cells immune system. that normally functions mitochondria harmless macroorganism. In this review, factors limiting ways increase it are discussed from point view properties cytC–carrier particles. different techniques used preparation cytC-bearing colloids discussed. Articles reporting achievement high each critically analyzed.

Language: Английский

Citations

0
Ultrasonication-Assisted Green Synthesis and Physicochemical and Cytotoxic Activity Characterization of Protein-Based Nanoparticles from Moringa oleifera Seeds DOI Creative Commons
Amany Abd El-Shafy Abd El-Kader Nafeh,

Ibrahim Mohamed Abd El-Aleem Mohamed,

Mohamed F. Foda

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(15), P. 1254 - 1254

Published: July 26, 2024

(

Citations

2
Nanoparticles-Delivered Circular RNA Strategy as a Novel Antitumor Approach DOI Open Access
Luisa Racca, Elisabetta Liuzzi,

Simona Comparato

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8934 - 8934

Published: Aug. 16, 2024

Anticancer therapy urgently needs the development of novel strategies. An innovative molecular target is represented by circular RNAs (circRNAs), single-strand RNA molecules with 5' and 3' ends joined, characterized a high stability. Although circRNA properties biological functions have only been partially elucidated, their relationship involvement in onset progression cancer emerged. Specific targeting circRNAs may be obtained antisense oligonucleotides silencing RNAs. Nanotechnology at forefront research for perfecting delivery. Continuous efforts made to develop nanoparticles (NPs) improve performance, materials, regarding biocompatibility capabilities. Applications various fields, from imaging gene therapy, explored. This review sums up smart strategies developed directly fruitful application NPs this context.

Language: Английский

Citations

2
Alternative Strategies for Delivering Immunotherapeutics Targeting the PD-1/PD-L1 Immune Checkpoint in Cancer DOI Creative Commons

Ryunosuke Hoshi,

Kristyna A. Gorospe,

Hagar I. Labouta

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(9), P. 1181 - 1181

Published: Sept. 7, 2024

The programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) immune checkpoint constitutes an inhibitory pathway best known for its regulation of cluster differentiation 8 (CD8)+ T cell-mediated responses. Engagement PD-L1 with PD-1 expressed on CD8+ cells activates downstream signaling pathways that culminate in cell exhaustion and/or apoptosis. Physiologically, these immunosuppressive effects exist to prevent autoimmunity, but cancer exploit this by overexpressing facilitate escape. Intravenously (IV) administered inhibitors (ICIs) block the interaction between PD-1/PD-L1 have achieved great success reversing and promoting tumor regression various malignancies. However, ICIs can cause immune-related adverse events (irAEs) due off-tumor toxicities which limits their therapeutic potential. Therefore, considerable effort has been channeled into exploring alternative delivery strategies enhance tumor-directed reduce irAEs. Here, we briefly describe PD-1/PD-L1-targeted immunotherapy associated We then provide a detailed review approaches, including locoregional (LDD)-, oncolytic virus (OV)-, nanoparticle (NP)-, ultrasound microbubble (USMB)-mediated are currently under investigation enhancing tumor-specific minimize toxic effects. conclude commentary key challenges methods potential mitigate them.

Language: Английский

Citations

2
Paclitaxel-Loaded, Pegylated Carboxylic Graphene Oxide with High Colloidal Stability, Sustained, pH-Responsive Release and Strong Anticancer Effects on Lung Cancer A549 Cell Line DOI Creative Commons
Athina Angelopoulou,

Myria Papachristodoulou,

Efstathia Voulgari

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(11), P. 1452 - 1452

Published: Nov. 14, 2024

Background: Graphene Oxide (GO) has shown great potential in biomedical applications for cancer therapeutics. The biosafety and stability issues of GO biological media have been addressed by functionalization with polyethylene glycol (PEG). Methods: In this work, carboxylated, nanosized (nCGO) was evaluated as a carrier paclitaxel (PCT). effect PEG characteristics on particle size surface charge, colloidal stability, drug, release, the hemolytic nCGO, investigated. Optimum PEG-nCGO/PCT formulations based above properties were their anticancer activity (cytotoxicity apoptosis induction) A549 lung cell line. Results: An increase length linear chains use branched (4-arm) instead resulted decrease hydrodynamic diameter an ζ pegylated nCGO particles. Pegylated exhibited high phosphate-buffered saline culture low effect, even at relatively concentration 1 mg/mL. molecular weight branching adversely affected PCT loading. increased rate release acidic pH 6.0 compared to physiological 7.4 observed all types nCGO/PCT. lower cytotoxicity apoptotic than non-pegylated nCGO. Cellular uptake incubation time cells leading time, which became higher that free 24 48 h incubation. Conclusions: biocompatibility enhanced are desirable regard clinical application nanomedicine.

Language: Английский

Citations

2
Sustainable Nanomedicine: Enhancement of Asplatin’s Cytotoxicity In Vitro and In Vivo Using Green-Synthesized Zinc Oxide Nanoparticles Formed via Microwave-Assisted and Gambogic Acid-Mediated Processes DOI Creative Commons
Hatem A. F. M. Hassan, Nada K. Sedky, Mohamed S. Nafie

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5327 - 5327

Published: Nov. 12, 2024

Chemoresistance encountered using conventional chemotherapy demands novel treatment approaches. Asplatin (Asp), a platinum (IV) prodrug designed to release cisplatin and aspirin in reductive environment, has demonstrated high cytotoxicity at reduced drug resistance. Herein, we investigated the ability of green-synthesized nanocarriers enhance Asp’s efficacy. Zinc oxide nanoparticles (ZnO-NPs) were synthesized green microwave-assisted method with reducing capping agent gambogic acid (GA). These then loaded Asp, yielding Asp@ZnO-NPs. Transmission electron microscopy was utilized study morphological features ZnO-NPs. Cell viability studies conducted on MDA-MB-231 breast cancer cells Asp@ZnO-NPs significantly decrease half-maximal inhibitory concentration (IC50) (5 ± 1 µg/mL). This further flow cytometric analysis that revealed capacity increase late apoptotic fractions. Furthermore, vivo carried out solid Ehrlich carcinoma-bearing mice showed significant tumor volume reduction (156.3 7.6 mm3), compared Asp alone (202.3 8.4 mm3) untreated controls (342.6 10.3 mm3). The histopathological increased necrosis Asp@ZnO-NPs-treated group. Asp@ZnO-NPs, an eco-friendly approach, enhanced anticancer activity, offering sustainable solution for potent formulations.

Language: Английский

Citations

1