Genome-Wide Identification and Expression Analysis of Gs and Gogat Gene Family in Pecan (<em>Carya illinoinensis</em>) under Different Nitrogen Forms

Published: Oct. 30, 2023

Nitrogen (N) is an important mineral nutrient for plant growth, as well a limiting factor crop yield, and how to improve the utilization efficiency of N fertilizer by plants currently research hotspot. This article uses bioinformatics methods identify analyze members glutamine synthetase (GS) glutamate synthase (GOGAT) gene families in pecan. A total 6 GS genes 4 GOGAT were identified, their physicochemical properties, structures, homologous evolutionary relationships analyzed. Analysis tissue-specific expression based on transcriptome data from The enzyme activities quantitatively analyzed under different form ratios According results, promoter cis-acting elements can be roughly divided into three types: light-responsive elements, hormone-responsive stress-responsive elements. results evolution showed that there was no tandem duplication event two families, have undergone purification during process. CiGS2s CiFd-GOGATs expressed mainly leaves, CiNADH-GOGATs fruits. qPCR analysis T4 treatment significantly increased levels CiGS CiGOGAT leaves. pecan T3, T4, T5 treatments. In summary, higher proportion ammonium nitrogen (NH4+) solution profit NH4+ assimilation. study determined appropriate ratio pecan, promoting theoretical basis reducing environmental pollution caused improving both CiGSs CiGOGATs exhibit tissue specificity, ammonium-nitrate mixture with more favorable assimilation provides reference further understanding functions offers foundation use

Language: Английский

Genomic organization and expression profiles of nitrogen assimilation genes in Glycine max

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17590 - e17590

Published: June 24, 2024

Background Glutamine synthetase (GS), glutamate synthase (GOGAT), and nitrate reductase (NR) are key enzymes involved in nitrogen assimilation metabolism plants. However, the systematic analysis of these gene families lacked reports soybean ( Glycine max (L.) Merr.), one most important crops worldwide. Methods In this study, we performed genome-wide identification characterization GS , GOGAT NR genes under abiotic stress conditions. Results We identified a total 10 genes, six four genome. Phylogenetic revealed presence multiple isoforms for each family, indicating their functional diversification. The distribution on chromosomes was uneven, with segmental duplication events contributing to expansion. Within (NAGs) group, there uniformity exon-intron structure conserved motifs NAGs. Furthermore, cis-elements NAG promoters indicated complex regulation expression. RT-qPCR seven NAGs various stresses, including deficiency, drought-nitrogen, salinity, distinct regulatory patterns. Most exhibited up-regulation stress, while diverse expression patterns were observed salt drought-nitrogen crucial role tolerance. These findings offer valuable insights into genomic organization profiles results have potential applications development stress-resistant varieties through genetic engineering breeding.

Language: Английский