Epitope Mapping of an Anti-Mouse CD39 Monoclonal Antibody using PA Scanning and RIEDL Scanning DOI Open Access
Hiroyuki Suzuki, Yuki Okada, Tomohiro Tanaka

et al.

Published: Dec. 6, 2023

A cell-surface ectonucleotidase CD39 mediates the conversion of extracellular ATP into immunosuppressive adenosine with another CD73. The elevated in tumor microenvironment (TME) attenuates antitumor immunity, which promotes cell immunologic escape and progression. Anti-CD39 monoclonal antibodies (mAbs), suppress enzymatic activity, can be applied to therapy. Therefore, an understanding relationship between inhibitory activity epitope mAbs is important. We previously established anti-mouse (mCD39) mAb, C39Mab-1 using Cell-Based Immunization Screening (CBIS) method. In this study, we determined critical flow cytometry. performed PA tag (12 amino acids)-substituted analysis (named scanning) RIEDL (5 determine By combination scanning scanning, identified conformational epitope, spanning three segments 275th 279th, 282nd 291st, 306th 323rd acids mCD39. These analyses would contribute identification membrane proteins.

Language: Английский

Epitope Mapping of an Anti-Mouse CD39 Monoclonal Antibody using PA Scanning and RIEDL Scanning DOI Open Access
Hiroyuki Suzuki, Yuki Okada, Tomohiro Tanaka

et al.

Published: Dec. 6, 2023

A cell-surface ectonucleotidase CD39 mediates the conversion of extracellular ATP into immunosuppressive adenosine with another CD73. The elevated in tumor microenvironment (TME) attenuates antitumor immunity, which promotes cell immunologic escape and progression. Anti-CD39 monoclonal antibodies (mAbs), suppress enzymatic activity, can be applied to therapy. Therefore, an understanding relationship between inhibitory activity epitope mAbs is important. We previously established anti-mouse (mCD39) mAb, C39Mab-1 using Cell-Based Immunization Screening (CBIS) method. In this study, we determined critical flow cytometry. performed PA tag (12 amino acids)-substituted analysis (named scanning) RIEDL (5 determine By combination scanning scanning, identified conformational epitope, spanning three segments 275th 279th, 282nd 291st, 306th 323rd acids mCD39. These analyses would contribute identification membrane proteins.

Language: Английский

Citations

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