Porcine β-defensin 114: Creating a Dichotomous Response to Inflammation DOI Open Access
Guoqi Su, Shaojun Huang, Shan Jiang

et al.

Published: Dec. 8, 2023

This study explored the effects of pBD114 (porcine β-defensin 114) on inflammatory response. The results in RAW264.7 cells showed that 100 μg/mL significantly increased concentrations TNFα and IL-10, 10 decreased concen-tration LPS-induced cell cultures at ng/mL. These suggest can exhibit pro-inflammatory or anti-inflammatory activities under certain conditions. RNA-seq analysis was performed to gain further insight into in-flammatory Among promoting response, up-regulated 1170 genes down-regulated 724 genes. KEGG enrichment found DEGs were enriched immune- signal-transduction-related signaling pathways. PPI KDA analyses revealed Bcl10 Bcl3 key In addition, 12 38 an-ti-inflammatory mainly en-riched "Cytokine-cytokine receptor interaction" pathway, Stat1 Csf2 qRT-PCR verified those RNA-seq. vivo mice tests also confirmed pro- activity pBD114. Although response is a rapid complex physiological reaction noxious stimuli, this plays an essential role by acting related immunity, signal transduction, molecules, interactions.

Language: Английский

Porcine β-defensin 114: Creating a Dichotomous Response to Inflammation DOI Open Access
Guoqi Su, Shaojun Huang, Shan Jiang

et al.

Published: Dec. 8, 2023

This study explored the effects of pBD114 (porcine β-defensin 114) on inflammatory response. The results in RAW264.7 cells showed that 100 μg/mL significantly increased concentrations TNFα and IL-10, 10 decreased concen-tration LPS-induced cell cultures at ng/mL. These suggest can exhibit pro-inflammatory or anti-inflammatory activities under certain conditions. RNA-seq analysis was performed to gain further insight into in-flammatory Among promoting response, up-regulated 1170 genes down-regulated 724 genes. KEGG enrichment found DEGs were enriched immune- signal-transduction-related signaling pathways. PPI KDA analyses revealed Bcl10 Bcl3 key In addition, 12 38 an-ti-inflammatory mainly en-riched "Cytokine-cytokine receptor interaction" pathway, Stat1 Csf2 qRT-PCR verified those RNA-seq. vivo mice tests also confirmed pro- activity pBD114. Although response is a rapid complex physiological reaction noxious stimuli, this plays an essential role by acting related immunity, signal transduction, molecules, interactions.

Language: Английский

Citations

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