Genome-wide analysis of XTH gene family in cucumber (Cucumis sativus) against different insecticides to enhance defense mechanism

Plant Stress, Journal Year: 2024, Volume and Issue: 13, P. 100538 - 100538

Published: July 19, 2024

This study investigates how plants protect themselves, specifically looking at a group of genes called Xyloglucan Endotransglucosylase/Hydrolase (XTH) in cucumber plants. A detailed investigation CsXTH genes, uncover their evolutionary background, structural motifs, regulatory networks, and functional responsibilities. The utilized various bioinformatics tools databases to highlight the dynamic interplay between abiotic stress responses. begins with phylogenetic analysis that reveals cladding into four categories. Further unraveling conserved motif domain analyses expose coordination xyloglucan modification, highlighting essential motifs domains shared among these genes. Analyzing cis-regulatory elements helps us understand complex networks control defense mechanisms Among elements, CGTCA-motif TGACG-motif stand out as key players interpreting environmental signals coordinating microRNA Csa-miR169 is becoming player regulating expression controlling biological responses Chromosome mapping, Ka/Ks calculations, protein-protein interaction offer insights dynamics Additionally, explores sub-cellular localization, uncovering diverse cellular compartments where are active. Finally, through chrysophanol physcion, we unveil adaptable functions specific response distinct treatments, underscoring role orchestrating cucumber's adaptive strategies.

Language: Английский

Genome-Wide Analysis of the Xyloglucan Endotransglucosylase/ Hydrolase (XTH) Gene Family: Expression Pattern During Magnesium Stress Treatment in the Mulberry Plant (<em>Morus alba L</em>)

Published: Dec. 12, 2023

Mulberry (Morus alba L), a significant fruit tree crop, requires magnesium (Mg) for its optimal growth and productivity. Nonetheless, our understanding of the molecular basis underlying stress tolerance in mulberry plants remains unexplored. In previous study, we identified several differential candidate genes associated with Mg homeostasis via transcriptome analysis including xyloglucan endotransglucosylase/hydrolase (XTH) gene family. The XTH family is crucial plant cell wall reconstruction responses. These have been thoroughly investigated various species. However, there no research pertaining to within M. plant. This systematically examined (MaXTH) at genomic level using bioinformatic approach. total, 22 MaXTH were discovered contained Glyco_hydro_16 XET_C conserved domains. MaXTH’s categorized into five distinct groups by their phylogenetic relationships. structure possesses four exons three introns. Furthermore, promoter reveals plethora cis-regulatory elements; mainly responsiveness, development, light phytohormone responsiveness. GO indicated that MaXTHs encode proteins exhibit xyloglucosyl transferase hydrolase activities addition biogenesis as well carbohydrate metabolic processes. Moreover, synteny unveiled an evolutionary relationship between those other species: A. thaliana, P. trichocarpa, Zea mays. Expression profiles from RNA-Seq data displayed expression patterns leaf tissue during treatments. Real-time quantitative PCR confirmed response. Overall, this enhances characteristics members lays foundation future functional study alba.

Language: Английский