An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota DOI Open Access
Jiangtao Zhou,

D. W. Li,

Jiajing Wang

et al.

Published: Dec. 26, 2023

Intestinal mucositis (IM) is a common adverse effect of chemotherapy, limiting its clinical application. Edible Chinese medicine, specifically Codonopsis pilosula-derived CP-A, with anti-inflammatory and gastrointestinal protective effects. The present work was to investigate CP-A's role in ameliorating IM mechanism using vitro rat models. Western blot, immunohistochemical (IHC) real-time PCR (RT-PCR) analyses were used assess protein expression related the extracellular-regulated kinases (ERK)/myosin light chain kinase (MLCK)/myosin 2 (MLC2) signaling pathway tight junction proteins. Enzyme-linked immunosorbent assay (ELISA) conducted measure inflammatory factors, 16S rRNA Amplicon Sequencing employed for cecum content analysis. results indicated that CP-A could restore body weight, food intake histopathological changes rats. Besides, abnormal MLCK activation induced by 5-fluorouracil (5-FU) attenuated via ERK/MLCK/MLC2 pathway. treatment improved levels reduced factor expression. On other hand, intestinal flora experiment demonstrated intervention increase abundance lactobacillus. In conclusion, mitigates 5-FU-induced inhibiting pathway, reducing improving mucosal barrier regulating flora. This study sheds on therapeutic potential treatment, providing insights future research.

Language: Английский

An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota DOI Open Access
Jiangtao Zhou,

D. W. Li,

Jiajing Wang

et al.

Published: Dec. 26, 2023

Intestinal mucositis (IM) is a common adverse effect of chemotherapy, limiting its clinical application. Edible Chinese medicine, specifically Codonopsis pilosula-derived CP-A, with anti-inflammatory and gastrointestinal protective effects. The present work was to investigate CP-A's role in ameliorating IM mechanism using vitro rat models. Western blot, immunohistochemical (IHC) real-time PCR (RT-PCR) analyses were used assess protein expression related the extracellular-regulated kinases (ERK)/myosin light chain kinase (MLCK)/myosin 2 (MLC2) signaling pathway tight junction proteins. Enzyme-linked immunosorbent assay (ELISA) conducted measure inflammatory factors, 16S rRNA Amplicon Sequencing employed for cecum content analysis. results indicated that CP-A could restore body weight, food intake histopathological changes rats. Besides, abnormal MLCK activation induced by 5-fluorouracil (5-FU) attenuated via ERK/MLCK/MLC2 pathway. treatment improved levels reduced factor expression. On other hand, intestinal flora experiment demonstrated intervention increase abundance lactobacillus. In conclusion, mitigates 5-FU-induced inhibiting pathway, reducing improving mucosal barrier regulating flora. This study sheds on therapeutic potential treatment, providing insights future research.

Language: Английский

Citations

1