Comparative Analysis of Malaxidinae (Orchidaceae) Plastome: Phylogenetic Relationship and Potential Molecular Marker DOI Open Access

Meng-Yao Zeng,

Ming-He Li,

Jingshan Shi

et al.

Published: Dec. 26, 2023

Malaxidinae is one of the confusing groups with classification in Orchidaceae. Previous phylogenetic analysis reveals that relationships among taxa have not yet been reliably established using a few plastome regions or nuclear ribosomal internal transcribed spacer (nrITS). In present study, complete plastomes Oberonia integerrima and Crepidium purpureum were assembled high-throughput sequencing. Combined publicly available data, this resulted data set 19 plastomes, including 17 species Malaxidinae. The feature relationship compared analyzed. results showed that: (1) possessed quadripartite structure typical angiosperms, sizes ranging from 142,996 bp to 158,787 encoding 125 133 genes. ndh genes lost pseudogenized varying degrees six species. An unusal inversion was detected Large Single-Copy region (LSC) Oberonioides microtatantha. (2) A total 8 regions, ycf1, matK, rps16, rpl32, ccsA-ndhD, clpP-psbB, trn-FGAA-ndhJ, trnSGCU-trnGUCC, identified as mutational hotspots. (3) Based on 68 protein-coding 51 intergenic respectively, analyses resolved genera-level subtribe strong support. Liparis supported polyphyletic.

Language: Английский

Comparative Analysis of Malaxidinae (Orchidaceae) Plastome: Phylogenetic Relationship and Potential Molecular Marker DOI Open Access

Meng-Yao Zeng,

Ming-He Li,

Jingshan Shi

et al.

Published: Dec. 26, 2023

Malaxidinae is one of the confusing groups with classification in Orchidaceae. Previous phylogenetic analysis reveals that relationships among taxa have not yet been reliably established using a few plastome regions or nuclear ribosomal internal transcribed spacer (nrITS). In present study, complete plastomes Oberonia integerrima and Crepidium purpureum were assembled high-throughput sequencing. Combined publicly available data, this resulted data set 19 plastomes, including 17 species Malaxidinae. The feature relationship compared analyzed. results showed that: (1) possessed quadripartite structure typical angiosperms, sizes ranging from 142,996 bp to 158,787 encoding 125 133 genes. ndh genes lost pseudogenized varying degrees six species. An unusal inversion was detected Large Single-Copy region (LSC) Oberonioides microtatantha. (2) A total 8 regions, ycf1, matK, rps16, rpl32, ccsA-ndhD, clpP-psbB, trn-FGAA-ndhJ, trnSGCU-trnGUCC, identified as mutational hotspots. (3) Based on 68 protein-coding 51 intergenic respectively, analyses resolved genera-level subtribe strong support. Liparis supported polyphyletic.

Language: Английский

Citations

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