Establishment of a Novel Anti-mouse CCR1 Monoclonal Antibody C<sub>1</sub>Mab-6 DOI Open Access
Tsunenori Ouchida,

Yu Isoda,

Tomohiro Tanaka

et al.

Published: Dec. 27, 2023

C-C motif chemokine receptor 1 (CCR1/CD191) is a member of G-protein-coupled receptors, and expressed on myeloid cells, such as neutrophils macrophages. Because the CCR1 signaling promotes tumor expansion in microenvironment (TME), modification TME an effective strategy for cancer therapy. Although attractive target solid tumors hematological malignancies, anti-CCR1 therapeutic agents have not been approved. Here, we established novel anti-mouse (mCCR1) monoclonal antibody (mAb), C1Mab-6 (rat IgG2b, kappa), using Cell-Based Immunization Screening (CIBS) method. Flow cytometry Western blot analyses showed that recognizes mCCR1 specifically. The dissociation constant mCCR1-overexpressed Chinese hamster ovary-K1 was determined 3.9&times;10-9 M, indicating possesses high affinity to mCCR1. These results suggest could be useful tool targeting preclinical mouse models.

Language: Английский

Development of a Sensitive Anti-Mouse CCR5 Monoclonal Antibody for Flow Cytometry DOI
Hiroyuki Suzuki, Tomohiro Tanaka, Guanjie Li

et al.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, Journal Year: 2024, Volume and Issue: 43(4), P. 96 - 100

Published: June 5, 2024

C-C chemokine receptor 5 (CCR5), a member of the G protein-coupled family, is most common coreceptor for human immunodeficiency virus type 1. CCR5 also involved in pathogenesis tumors and inflammatory diseases. The antagonists including monoclonal antibodies (mAbs) have been developed evaluated clinical trials. In this study, we novel mAbs mouse (mCCR5) using Cell-Based Immunization Screening (CBIS) method. One established anti-mCCR5 mAbs, C

Language: Английский

Citations

5
Epitope Mapping of an Anti-mouse CCR8 Monoclonal Antibody C<sub>8</sub>Mab-2 Using Flow Cytometry DOI Open Access

Hiyori Kobayashi,

Hiroyuki Suzuki, Tomohiro Tanaka

et al.

Published: Jan. 26, 2024

The C-C motif chemokine receptor 8 (CCR8) is highly and selectively expressed in regulatory T (Treg) cells associated with tumor progression. massive accumulation of Treg into tumors suppresses the effector function CD8+ against cells. Therefore, selective depletion using anti-CCR8 monoclonal antibodies (mAbs) reinvigorates antitumor immune responses improves to cancer immunotherapy. Previously, we developed an anti-mouse CCR8 (mCCR8) mAb, C8Mab-2, Cell-Based Immunization Screening (CBIS) method. In this study, binding epitope C8Mab-2 was investigated flow cytometry. mCCR8 extracellular domain-substituted mutant analysis showed that recognizes N-terminal region (1&ndash;33 amino acids) mCCR8. Next, 1&times;alanine (or glycine) scanning 2&times;alanine were conducted region. results revealed 17-DFFTAP-22 sequence important for recognition by Thr20 a central acid epitope. These involvement N-terminus C8Mab-2.

Language: Английский

Citations

1
Establishment of a Novel Anti-mouse CCR1 Monoclonal Antibody C<sub>1</sub>Mab-6 DOI Open Access
Tsunenori Ouchida,

Yu Isoda,

Tomohiro Tanaka

et al.

Published: Dec. 27, 2023

C-C motif chemokine receptor 1 (CCR1/CD191) is a member of G-protein-coupled receptors, and expressed on myeloid cells, such as neutrophils macrophages. Because the CCR1 signaling promotes tumor expansion in microenvironment (TME), modification TME an effective strategy for cancer therapy. Although attractive target solid tumors hematological malignancies, anti-CCR1 therapeutic agents have not been approved. Here, we established novel anti-mouse (mCCR1) monoclonal antibody (mAb), C1Mab-6 (rat IgG2b, kappa), using Cell-Based Immunization Screening (CIBS) method. Flow cytometry Western blot analyses showed that recognizes mCCR1 specifically. The dissociation constant mCCR1-overexpressed Chinese hamster ovary-K1 was determined 3.9&times;10-9 M, indicating possesses high affinity to mCCR1. These results suggest could be useful tool targeting preclinical mouse models.

Language: Английский

Citations

3