Rapid Molecular Diagnostics of Pneumonia Caused by Gram-Negative Bacteria: A Clinician’s Review DOI Creative Commons
Ionela-Anca Pintea-Simon, Ligia Ariana Bancu, Anca Delia Mare

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 805 - 805

Published: Aug. 25, 2024

With approximately half a billion events per year, lower respiratory tract infections (LRTIs) represent major challenge for the global public health. Among LRTI cases, those caused by Gram-negative bacteria (GNB) are associated with poorer prognostic. Standard-of-care etiologic diagnostics is lengthy and difficult to establish, more than of cases remaining microbiologically undocumented. Recently, syndromic molecular diagnostic panels became available, enabling simultaneous detection tens pathogen-related antimicrobial-resistance genetic markers within few hours. In this narrative review, we summarize available data on performance in GNB pneumonia, highlighting main strengths limitations these assays, as well factors influencing their clinical utility. We searched MEDLINE Web Science databases relevant English-language articles. Molecular assays have higher analytical sensitivity cultural methods, show good agreement standard-of-care regarding pathogens, including GNB, identification frequent patterns resistance antibiotics. Clinical trials reported encouraging results usefulness antibiotic stewardship. By providing early information presence pathogens probable phenotypes, assist choice targeted therapy, shortening time from sample collection appropriate antimicrobial treatment, reducing unnecessary use.

Language: Английский

FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols DOI Open Access
Stephen A. Bustin,

Sara Kirvell,

Tania Nolan

et al.

Published: Jan. 18, 2024

Versatility, sensitivity and accuracy have made the real-time polymerase chain reaction (qPCR) a crucial tool for research as well diagnostic applications. However, point-of-care (PoC) use traditional qPCR faces two main challenges: long run times mean results are not available half an hour or more requisite high-temperature denaturation requires robust pow-er-demanding instrumentation. This study addresses both issues revised primer probe designs, modified buffers low ∆T protocols which, together, speed up on conventional instruments will allow development of robust, devices. Our approach, called "FlashPCR", also allows efficient reverse transcription part one-step RT-qPCR pro-tocol, making it universally applicable rapid

Language: Английский

Citations

2
Rapid Molecular Diagnostics of Pneumonia Caused by Gram-Negative Bacteria: A Clinician’s Review DOI Creative Commons
Ionela-Anca Pintea-Simon, Ligia Ariana Bancu, Anca Delia Mare

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 805 - 805

Published: Aug. 25, 2024

With approximately half a billion events per year, lower respiratory tract infections (LRTIs) represent major challenge for the global public health. Among LRTI cases, those caused by Gram-negative bacteria (GNB) are associated with poorer prognostic. Standard-of-care etiologic diagnostics is lengthy and difficult to establish, more than of cases remaining microbiologically undocumented. Recently, syndromic molecular diagnostic panels became available, enabling simultaneous detection tens pathogen-related antimicrobial-resistance genetic markers within few hours. In this narrative review, we summarize available data on performance in GNB pneumonia, highlighting main strengths limitations these assays, as well factors influencing their clinical utility. We searched MEDLINE Web Science databases relevant English-language articles. Molecular assays have higher analytical sensitivity cultural methods, show good agreement standard-of-care regarding pathogens, including GNB, identification frequent patterns resistance antibiotics. Clinical trials reported encouraging results usefulness antibiotic stewardship. By providing early information presence pathogens probable phenotypes, assist choice targeted therapy, shortening time from sample collection appropriate antimicrobial treatment, reducing unnecessary use.

Language: Английский

Citations

0