Published: Feb. 1, 2024
Caused by pathogenic microorganisms, such as bacteria, parasites, fungi, among others, infectious diseases can spread directly or from one individual to another. According the World Health Organization, these are known cause high mortality rates, severe burdens of disability and serious worldwide aftermaths. The inappropriate use antibiotics in humans main origins development drug-resistant pathogens, which reduce efficacy therapies, thus accentuating need search for effective antimicrobials. Medicinal plants have served starting material preparation a number antimicrobial agents. To this end, present study highlights green synthesis Cocos nucifera-based nanomaterials evaluation mechanistic basis their action. Accordingly, nucifera extract was used different concentrations (5, 10 20 mM) silver nitrate solution afford nanoparticles (AgNPs). These entities were further incorporated onto activated carbons (obtained chemical activation using sulphuric acid agent) generate nanocomposites. activity as-prepared evaluated broth microdilution method, while, antioxidant assessed through standard methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic (ABTS) assays. Cytotoxicity potent on Vero cells spectrophotometric method. As result, successfully synthesized evidenced color change transparent dark brown. Further characterization carried out UV-visible spectroscopy that showed an intense absorption spectrum at 433 nm. Fourier Transform Infrared Spectroscopy (FTIR) revealed functional group moieties involved capping reducing agent AgNPs. incubation with selected bacterial fungal strains led significant inhibitory effects pathogens minimum ranging 7.813 250 μg/ml. In assays, nanocomposites presented scavenging activities comparable ascorbic acid. experiment no toxic human mammalian (range selectivity indices: >4 >128). results provide evidence implication targeting systems mediate free-radical damage inhibiting oxidative caused bacteria most susceptible being Escherichia coli Candida albicans, respectively.
Language: Английский