Colostrum-Derived Melatonin Plus PEG Microspheres Modulate the Oxidative Metabolism of Human Colostrum Phagocytes

Metabolites, Journal Year: 2025, Volume and Issue: 15(1), P. 57 - 57

Published: Jan. 16, 2025

Background/Objectives: Exogenous melatonin adsorbed onto PEG microspheres can modulate the functional activity of phagocytes in colostrum, but no data are available on found colostrum. Therefore, objective this study was to extract from human develop and characterize with extracted them, evaluate effects system oxidative metabolism colostrum phagocytes. Methods: Thirty samples were collected; ten used for extraction, while twenty obtain Melatonin supernatant through affinity chromatography quantified by ELISA. The polyethylene glycol produced analyzed using fluorescence microscopy flow cytometry. Oxidative assessed measuring release superoxide anion enzymes. A control conducted commercial melatonin. Results: cytometry analyses demonstrated that adsorb There an increase incubated colostrum-derived or synthetic When exposed bacteria, treated exhibited increased superoxide, accompanied a decrease dismutase (SOD) lower SOD-to-superoxide ratio. In contrast, reduced enzyme Conclusions: These highlight importance cellular suggest may be more effective option controlling metabolism, particularly during infectious processes.

Language: Английский

Antiviral Effect of Melatonin on Caco-2 Cell Organoid Culture: Trick or Treat?

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11872 - 11872

Published: Nov. 5, 2024

Melatonin is a hormone naturally produced by the body that has recently been found to have antiviral properties. However, its mechanisms are not entirely understood. Using Caco-2 cells, we developed gastrointestinal organoid model investigate impact of melatonin on cellular culture response Poly I:C-induced viral inflammation in tract. was different effect when applied as pretreatment before induction or treatment after it. I:C stimulation did protect organoids from size reduction but enhanced cell proliferation, especially lower (1 and 10 µM) concentrations were used. On other hand, with helped maintain while reducing proliferation. In pretreated reduced IFNLR1 expression found, increased production cytokines, such IFNλ1 STAT1-3, prevent apoptosis. The findings this study emphasize importance type III IFNs defense epithelial cells shed more light properties potential therapeutic substance.

Language: Английский

Melatonin Treatment at Dry-off Reduces Postpartum Shedding of Coccidia in Primiparous Dairy Cows and Their Calves

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3534 - 3534

Published: Dec. 6, 2024

Coccidiosis is a protozoan disease that causes diarrhea in cattle. This study examines the impact of treating pregnant cows at dry-off with melatonin on postpartum coccidia excretion dams and their calves. The population comprised 106 primiparous lactating dairy cows: 53 controls receiving days 220–226 gestation, plus 99 calves: 52 born from control 47 treated dams. Feces samples were collected each dam gestation 10–16 30–36 postpartum; calf age. Postpartum rates high oocysts per gram (OPG) (feces counts > 5000) significantly lower (p < 0.01) than Low OPG (<2000) higher treatment groups lactation calves life. In conclusion, reduced shedding during early period.

Language: Английский