Aquilaria crassna Extract Exerts Neuroprotective Effect against Benzo[a]pyrene-Induced Toxicity in Human SH-SY5Y Cells: An RNA-Seq-Based Transcriptome Analysis DOI Open Access
Nattaporn Pattarachotanant,

Suporn Sukjamnong,

Panthakarn Rangsinth

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2727 - 2727

Published: Aug. 16, 2024

Benzo[a]pyrene (B[a]P) is known to inhibit neurodifferentiation and induce neurodegeneration. Agarwood or Aquilaria crassna (AC), a plant with health-promoting properties, may counteract the neurotoxic effects of B[a]P by promoting neuronal growth survival. This study investigated protective effect AC leaf ethanolic extract (ACEE) on B[a]P-induced impairment differentiation. A transcriptomic analysis identified canonical pathway, biological network, differentially expressed genes (DEGs) that are changed in response differentiation neurogenesis. Several genes, including CXCR4, ENPP2, GAP43, GFRA2, NELL2, NFASC, NSG2, NGB, BASP1, NEUROD1, B[a]P-treated SH-SY5Y cells were up-regulated after treatment ACEE. Notably, Western blot further confirmed ACEE increased protein levels GAP43 neuroglobin. led decreased phosphorylation Akt ERK cells; however, was able reverse these effects. Clionasterol lupenone Molecular docking showed two phytochemicals had significant interactions GDNF family receptor alpha (GFRA), retinoid X receptors (RXRs). In conclusion, be potential alternative medicine for prevention impaired neurodegenerative diseases.

Language: Английский

A Survey on Computational Methods in Drug Discovery for Neurodegenerative Diseases DOI Creative Commons

Caterina Vicidomini,

Francesco Fontanella, Tiziana D’Alessandro

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1330 - 1330

Published: Oct. 19, 2024

Currently, the age structure of world population is changing due to declining birth rates and increasing life expectancy. As a result, physicians worldwide have treat an number age-related diseases, which neurological disorders represent significant part. In this context, there urgent need discover new therapeutic approaches counteract effects neurodegeneration on human health, computational science can be pivotal importance for more effective neurodrug discovery. The knowledge molecular receptors other biomolecules involved in pathogenesis facilitates design molecules as potential drugs used fight against diseases high social relevance such dementia, Alzheimer's disease (AD) Parkinson's (PD), cite only few. However, absence comprehensive guidelines regarding strengths weaknesses alternative creates fragmented disconnected field, resulting missed opportunities enhance performance achieve successful applications. This review aims summarize some most innovative strategies based methods development. particular, recent applications state-of-the-art docking artificial intelligence ligand- target-based novel drug were reviewed, highlighting crucial role silico context discovery neurodegenerative diseases.

Language: Английский

Citations

4

Drug Development for Alzheimer’s and Parkinson’s Disease: Where Do We Go Now? DOI Open Access
Lisa Sequeira, Sofia Benfeito, Carlos Fernandes

et al.

Published: April 5, 2024

Neurodegenerative diseases (NDs) are a set of progressive, chronic, and incurable characterized by the gradual loss neurons, culminating in decline cognitive and/or motor functions. Alzheimer’s disease (AD) Parkinson’s (PD) most common NDs represent an enormous burden both terms human suffering economic cost. The available therapies for AD PD only provide symptomatic palliative relief limited period unable to modify diseases’ progression. Over last decades, research efforts have been focused on developing new pharmacological treatments these NDs. However, date, no breakthrough treatment has discovered. Hence, development disease-modifying drugs able halt or reverse progression remains unmet clinical need. This review summarizes major hallmarks treatment. It also sheds light potential directions that can be pursued develop new, treat PD, thereby decreasing social burdens linked

Language: Английский

Citations

3

Aquilaria crassna Extract Exerts Neuroprotective Effect against Benzo[a]pyrene-Induced Toxicity in Human SH-SY5Y Cells: An RNA-Seq-Based Transcriptome Analysis DOI Open Access
Nattaporn Pattarachotanant,

Suporn Sukjamnong,

Panthakarn Rangsinth

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2727 - 2727

Published: Aug. 16, 2024

Benzo[a]pyrene (B[a]P) is known to inhibit neurodifferentiation and induce neurodegeneration. Agarwood or Aquilaria crassna (AC), a plant with health-promoting properties, may counteract the neurotoxic effects of B[a]P by promoting neuronal growth survival. This study investigated protective effect AC leaf ethanolic extract (ACEE) on B[a]P-induced impairment differentiation. A transcriptomic analysis identified canonical pathway, biological network, differentially expressed genes (DEGs) that are changed in response differentiation neurogenesis. Several genes, including CXCR4, ENPP2, GAP43, GFRA2, NELL2, NFASC, NSG2, NGB, BASP1, NEUROD1, B[a]P-treated SH-SY5Y cells were up-regulated after treatment ACEE. Notably, Western blot further confirmed ACEE increased protein levels GAP43 neuroglobin. led decreased phosphorylation Akt ERK cells; however, was able reverse these effects. Clionasterol lupenone Molecular docking showed two phytochemicals had significant interactions GDNF family receptor alpha (GFRA), retinoid X receptors (RXRs). In conclusion, be potential alternative medicine for prevention impaired neurodegenerative diseases.

Language: Английский

Citations

1