Toxicology International, Journal Year: 2025, Volume and Issue: unknown, P. 171 - 179
Published: March 12, 2025
Hemorrhagic septicemia caused by Aeromonas hydrophila is a major bacterial infection threatening sustainable aquaculture, particularly in species like Nile Tilapia (Oreochromis niloticus). Building on previous computational studies that identified quercetin as potential antibacterial agent against hydrophila, this study validates quercetin’s efficacy fish cell lines examining multifaceted approach, conducted FTIR to confirm the presence of and its functional group, evaluated impact DNA gyrase supercoiling inhibition cytotoxicity assessment line using MTT assay. Our findings show 80 μg/mL safe for OnIL niloticus liver line). Whereas 50, 60, 70, μg/mL, reveal potent inhibitory effects cultured with cells. This establishes effectiveness indicates could be approach managing hemorrhagic aquaculture. Major Findings: 1) The investigates use quercetin, natural polyphenol, pathogen responsible septicemia. 2) Quercetin concentrations showed strong A. when cells while at concentration was found Oreochromis (OnIL). 3) confirmed quercetin's antimicrobial activity through ability inhibit
Language: Английский