WRKY Transcription Factor Responses and Tolerance to Abiotic Stresses in Plants

Published: June 3, 2024

Plants are subjected to abiotic stresses throughout their developmental period. Abiotic include drought, salt, heat, cold, heavy metals, nutritional element and oxidative stresses. Improving plant response various environmental is critical for survival perpetuation. The WRKY transcription factors have special structure (WRKY structural domains), which enable different transcriptional regulatory functions. can not only regulate growth development by regulating phytohormone signalling pathways, but also promote or suppress the expression of downstream genes binding W-box [TGACCA/TGACCT] in promoters target genes. In addition, interact with other families defence responses stresses, self-regulate recognizing W-boxes own However, recent years, research reviews on roles higher plants scarce shallow. this review, we focus classification factors, as well identification molecular mechanisms involved improve tolerance ability stress look forward future directions, a view providing theoretical support genetic improvement crop tolerance.

Language: Английский

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Identification and Characterization of SQUAMOSA Promoter Binding Protein-like Transcription Factor Family Members in Zanthoxylum bungeanum and Their Expression Profiles in Response to Abiotic Stresses

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 520 - 520

Published: Feb. 8, 2025

Plant-specific transcription factors known as SQUAMOSA promoter binding protein-like (SPL) genes are essential for development, growth, and abiotic stress responses. While the SPL gene family has been extensively studied in various plant species, a systematic characterization Zanthoxylum bungeanum (Zb) is lacking. This study used transcriptomic bioinformatics data to conduct thorough genomic identification expression investigation of ZbSPL family. Eight subfamilies including 73 members were identified, most which predicted be localized nucleus. Ka/Ks ratio analysis indicates that have undergone purifying selection. According evolutionary research, segmental duplication major factor amplification Gene structures, conserved motifs, domains found highly among paralogs. Cis-element research revealed ZbSPLs may implicated hormone Codon usage pattern showed was more inclined A/T base endings; higher content, stronger preference codons; use mainly affected by natural Additionally, 36 potential targets miR156. RNA-seq demonstrated Zb differentially expressed response distinct stressors. Two (ZbSPL10 ZbSPL17) salt stress, while four (ZbSPL06, ZbSPL43, ZbSPL60, ZbSPL61) drought based on qRT-PCR under conditions. will help us gain deeper understanding functions lay genetic foundation future breeding high-quality, resistant varieties Z. bungeanum.

Language: Английский

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CsWRKY46 Is Involved in the Regulation of Cucumber Salt Stress by Regulating Abscisic Acid and Modulating Cellular Reactive Oxygen Species

Horticulturae, Journal Year: 2025, Volume and Issue: 11(3), P. 251 - 251

Published: Feb. 26, 2025

Soil salinity significantly restricts the growth, development, and productivity of vegetables. Cucumber, a crucial greenhouse vegetable, is helpful for understanding how plants perceive, signal, respond to salt stress. The WRKY family plays an important role in regulating stress responses. This study utilized cucumber variety ‘Zhongnong 26’ investigate effects on morphological changes, physiological biochemical indices, molecular regulations. CsWRKY46 was up-regulated both ABA response conditions leaves, roots, fruits cucumber. Transgenic Arabidopsis lines overexpressing (CsWRK46-OE1 CsWRK46-OE5) showed higher proline accumulation reduced electrolyte leakage compared wild type (WT). These overexpression demonstrated peroxidase (POD) glutathione reductase (GR) activity, along with lower ascorbate (APX) catalase (CAT) activity. qRT-PCR analysis revealed elevated expression levels ABI5 ABF4 CsWRKY46-OE WT. Additionally, increased stress-inducible genes such as PSCS1, PY19, RD19. findings suggest that enhances plant tolerance stress, potentially through regulation modulation cellular reactive oxygen species (ROS), provide foundation identification new sources breeding programs.

Language: Английский

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Genome-Wide Analysis of CPP Transcription Factor Family in Endangered Plant Phoebe bournei and Its Response to Adversity

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 803 - 803

Published: March 5, 2025

The CPP gene family comprises transcription factor genes containing a conserved CRC domain, which is mainly involved in plant development and evolution. Although have been widely studied many plants, little known about them woody especially the endangered species Phoebe bournei (Hemsl.). In genome of bournei, we identified 11 PbCPP (PbCPP1-PbCPP11) distributed on four chromosomes, with large differences number amino acids. They encode both acidic alkaline proteins. A phylogenetic analysis showed that these can be divided into three subfamilies, A, B, C, contain seven, two, two genes, respectively. Through an interspecific collinearity analysis, homologous genes. promoter cis-acting element revealed PbCPPs variety elements respond to hormones, stress signals, light play role growth development, most (except PbCPP3 PbCPP8) MYB binding site regulate drought-induced responses, indicating they important drought resistance. An expression PbCPP4 was high roots stems lower leaves, whereas other low roots, stems, leaves. addition, six representative were detected using qRT-PCR. results show significant under abiotic conditions (drought, cold, salt), responses. This study preliminarily verified different great significance for understanding its mechanism adaptation.

Language: Английский

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Genome-Wide Identification of GLK Family Genes in Phoebe bournei and Their Transcriptional Analysis Under Abiotic Stresses

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2387 - 2387

Published: March 7, 2025

GOLDEN2-LIKE (GLK) transcription factors are crucial regulators of chloroplast development and stress responses in plants. In this study, we investigated the GLK gene family Phoebe bournei (Hemsl.) Yen C. Yang, a near-threatened species important for forestry wood utilization China. We identified 61 PbGLK genes which were classified into seven subfamilies. Our analyses their phylogenetic relationships, structures, chromosomal distribution revealed diverse characteristics. Expression profiling under different tissues abiotic stresses showed that PbGLK25 PbGLK30 particularly responsive to drought, heat, light, shade stresses, with significant upregulation. These findings highlight potential role adaptation provide insights genetic improvement P. bournei.

Language: Английский

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Molecular Insights into the Positive Role of Soybean Nodulation by GmWRKY17

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2965 - 2965

Published: March 25, 2025

Soybean is an important economic oilseed crop, being rich in protein and plant oil, it widely cultivated around the world. Soybeans have been shown to establish a symbiotic nitrogen fixation (SNF) with their compatible rhizobia, resulting formation of nodules. Previous studies demonstrated critical roles phytohormones, such as abscisic acid cytokinin, process legume nodulation. The present study investigated role GmWRKY17, homolog Rosa hybrida (Rh)WRKY13 regulating immunity through cytokinin content signaling soybean Utilizing real-time PCR histochemical staining, we that GmWRKY17 predominantly expressed root Subsequently, analyzed function GmWRKY17-overexpression, RNA interference (RNAi), CRISPR/Cas9 system. Overexpression significantly increases nodule number, while RNAi or CRISPR/Cas9-mediated knockout resulted dramatic repression soybeans. These results highlight functions positive regulator involved Furthermore, manipulation expression impacts genes associated nod factor (NF) pathway, thereby influencing This WRKY-type transcription factors are regulation nodulation, offering new light on molecular basis interaction between legumes rhizobia.

Language: Английский

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Transcriptomic Analysis of Leaves from Two Maize Hybrids Under Heat Stress During the Early Generative Stage

Genes, Journal Year: 2025, Volume and Issue: 16(5), P. 480 - 480

Published: April 24, 2025

Background: High temperatures during the early generative stage significantly threaten maize productivity, yet molecular basis of heat tolerance remains unclear. Methods: To elucidate mechanisms in maize, two hybrids—ZD309 (heat-tolerant) and XY335 (heat-sensitive)—were selected for integrated transcriptomic physiological analyses. The plants were subjected to high-temperature treatments (3–5 °C above ambient field temperature) 0, 1, 3, 5, 7 days, with controls grown under natural conditions. Physiological indices, including Superoxide dismutase (SOD) activity, proline (PRO), malondialdehyde (MDA), soluble sugar, protein content, measured. Results: Transcriptome analysis identified 1595 differentially expressed genes (DEGs) (509 up- 1086 down-regulated) 1526 DEGs ZD309 (863 663 down-regulated), most pronounced changes occurring on day 5. Key enriched galactose metabolism carbohydrate catabolism, whereas exhibited rapid activation oxidative stress cell wall integrity pathways. Mfuzz time-series categorized from into six clusters each. Weighted gene co-expression network (WGCNA) 10 hub involved ubiquitin thioesterase activity RNA modification, suggesting protein-level regulatory roles. Conclusions: This study reveals distinct transcriptional dynamics between heat-tolerant heat-sensitive varieties, providing candidate breeding thermotolerant advancing our understanding responses critical reproductive stages.

Language: Английский

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Plant Responses and Adaptations to Salt Stress: A Review

Horticulturae, Journal Year: 2024, Volume and Issue: 10(11), P. 1221 - 1221

Published: Nov. 18, 2024

Salinity poses a significant environmental challenge, limiting plant growth and development. To cultivate salt-tolerant plants, it is crucial to understand the physiological, biochemical, molecular responses adaptations salt stress, as well explore natural genetic resources linked tolerance. In this review, we provide detailed overview of mechanisms behind morphological physiological triggered by including damage disturbance cell osmotic potentials ion homeostasis, lipid peroxidation, suppression photosynthesis growth. We also describe that confer tolerance in such adjustments, reactive oxygen species (ROS) scavenging, photosynthetic responses, phytohormone regulation, regulation. Additionally, summarize salt-stress sensing signaling pathways, gene regulatory networks, salt-tolerance plants. The key pathways involved signal perception transduction, Ca2+-dependent protein kinase (CDPK) cascades, overly sensitive (SOS) pathway, abscisic acid (ABA) are discussed, along with relevant salt-stress-responsive genes transcription factors. end, important issues challenges related for future research addressed. Overall, review aims essential insights cultivation breeding crops fruits.

Language: Английский

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De Novo Transcriptome Assembly of Rice Bean (Vigna umbellata) and Characterization of WRKY Transcription Factors Response to Aluminum Stress

Plants, Journal Year: 2024, Volume and Issue: 13(22), P. 3170 - 3170

Published: Nov. 12, 2024

Rice bean is an underutilized legume crop cultivated in Asia, and it a good source of protein, minerals, essential fatty acids for human consumption. Moreover, the leaves left over after harvesting rice seeds contain various biological constituents beneficial to humans animals. In our study, we performed de-novo transcriptome assembly bean, characterized WRKY transcription factors, studied their response aluminum stress. A total 46.6 million clean reads, with GC value 43%, were generated via sequencing. De novo reads resulted 90,933 transcripts 74,926 unigenes, minimum maximum lengths 301 bp 24,052 bp, N50 values 1801 1710 respectively. 27,095 28,378 unigenes annotated subjected GO KEGG analyses. Among 15,593, 20,770, 15,385 identified domains process, molecular function, cellular component, 16,132 assigned 188 pathways, including metabolic pathways (5500) secondary metabolite biosynthesis (2858). Transcription factor analysis revealed 4860 from 98 different families. For WRKY, 95 identified. Further diverse factors Collectively, results this study boost genomic resources provide baseline further research on role tolerance bean.

Language: Английский

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Uncovering Key Transcription Factors Driving Chilling Stress Tolerance in Rice Germination

DNA, Journal Year: 2024, Volume and Issue: 4(4), P. 582 - 598

Published: Dec. 16, 2024

Background: Rice, one of the main foods in Brazil and world, is sensitive to chilling (0–15 °C), especially germination reproductive stages. Chilling causes delayed affects coleoptile elongation at S3 stage (needlepoint), causing poor plant establishment, stunted growth, non-vigorous plants, also impacting weed management. Elucidating mechanisms responsible for resilience under cold conditions helps development tolerant cultivars. Transcription factors (TFs) act stress response signaling, making them indispensable tolerance mechanism. Objective: Thus, this study aimed identify characterize expression profile transcription rice stage. Methods: To determine transcriptional 2408 genes belonging 56 TF families, RNAseq was performed on shoot tissue seedlings Oro (chilling-tolerant) Tio Taka (chilling-sensitive) genotypes grown control (25 °C) (13 until Results: Of total analyzed, 22% showed significant differential analyzed There were 117 that cultivar, 60 which downregulated 57 upregulated. In 248 had a expression, 98 150 A 170 encoding TFs commonly significantly differentially expressed genotypes. Conclusions: Here, we revealed potential new targets involved regulation

Language: Английский

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