Genetic Variants in the NOD-like Receptor Signaling Pathway Are Associated with HIV-1/AIDS in a Northern Chinese Population

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3484 - 3484

Published: April 8, 2025

The NOD-like receptor (NLR) signaling pathway may influence human immunodeficiency virus (HIV) clearance and CD4+ T cell recovery through inflammatory responses, but its specific mechanism requires further investigation. A deeper understanding of genetic variations can provide new insights into the biological mechanisms underlying occurrence development syndrome (AIDS). By utilizing multiple bioinformatic analyses functional annotations, we identified single-nucleotide polymorphisms (SNPs) in NLR that affect HIV-1 infection AIDS progression. Then, a case-control study was performed to screen risk-related variants by genotyping candidate SNPs sample 500 men who have sex with (MSM) healthy controls from Han population Northern China. results revealed significant association between five (NLRP3 rs4612666, MAVS rs17857295, rs6084497, rs16989000, JAK1 rs4244165) infection. Interestingly, gene-gene interaction model composed exhibited cumulative effect on disease. Specially, increase risk alleles carried samples elevated contracting HIV-1. In addition, three (IL1B rs1143623, STAT1 rs1467199 rs2066804) were associated counts patients AIDS. Three (OAS1 rs1131454, NLRP3 rs10754558, rs867335) found be related clinical staging This finding provides genes progression among MSM

Language: Английский

Downregulation of miRNA-26a by HIV-1 Enhances CD59 Expression and Packaging Impacting Virus Susceptibility to Antibody-Dependent Complement-Mediated Lysis

Published: June 5, 2024

MicroRNAs (miRNAs) play important roles in the control of HIV-1 infection. Here, we performed RNAseq profilings miRNAs and mRNAs expressed CD4+ T-lymphocytes upon Our results reveal significant alterations expression profiles infected relative to uninfected cells. One markedly downregulated cells is miRNA-26a. Among putative targets miRNA-26a are CD59 receptor transcripts, which significantly upregulated T-cells. Addition mimics T-cells reduces at both mRNA surface protein levels, validating as a target. Consistent with reported inhibitory role complement-mediated lysis (CML), knocking-out renders progeny virions more prone antibody-dependent CML (ADCML). leads enhanced sensitivity ADCML, condition linked reduction packaging into released virions. Lastly, HIV-1-mediated downregulation shown be dependent on integrated but does not involve viral accessory proteins. Overall, these highlight novel mechanism by limits ADCML upregulating via downmodulation.

Language: Английский