Ratanjot (<em>Alkanna tinctoria</em> L.) Root Extract, Rich in Antioxidants, Exhibits Strong Antimicrobial Activity Against Foodborne Pathogens and Is a Potential Food Preservative

Published: June 23, 2024

Natural and sustainable plant-based antioxidants antimicrobials are highly desirable for improving food quality safety. The present investigation assessed the antimicrobial antioxidant properties of active components from Alkanna tinctoria (herb) roots, also known as Ratanjot root. Two methods were used to extract components: microwave-assisted hot water (MAHW) ethanol air-dried. MAHW yielded 6.29% while 18.27%, suggesting superior root powder (RRP) solubility in over water. showed significantly higher activity than extract. Gas chromatography mass-spec analysis revealed three major phenolic compounds: Butanoic acid, 3-hydroxy-3-methyl-; Arnebin 7, Diisooctylpthalate. color attributes (L*, a*, b*, hab, C*ab) ethanolic extracts significant differences (P &lt; 0.05) all above parameters both types extracts, except yellowness (b*) chroma (C*ab) values. exhibited against 14 bacterial species, with a signifi-cantly inhibitory effect Gram-positive bacteria (Listeria monocytogenes Staphylococcus aureus) Gram-negative (Salmonella enterica serovar Typhimurium Escherichia coli). minimum concentration (MIC) bactericidal (MBC) 25 mg/ml bacteria, MIC MBC concentrations varied (0.049 - 0.098 0.195 mg/ml) was bactericidal. activities RRP remained stable under broad temperature (37-100°C) pH (2-6) conditions, well during refrigerated storage 30 days. Application at 1% (10 mg/g) 2.5% (25 levels cooked chicken meatball model system prevented lipid oxidation improved sensory retarded microbial growth (4°C) 20 Furthermore, non-toxic when tested sheep erythrocytes did not inhibit probiotics, Lacticaseibacillus casei, Lactiplantibacillus plantarum. In conclusion, study suggests that possesses excellent activities, thus making it suitable preservation.

Language: Английский

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Comparison of the Potential of “Green” Classical and Natural Deep Eutectic Solvents in the Production of Natural Food Colorant Extracts from the Roots of Alkanna tinctoria (L.)

Foods, Journal Year: 2025, Volume and Issue: 14(4), P. 584 - 584

Published: Feb. 10, 2025

Alkanna tinctoria L. Tausch ("alkanet" or "alkanna") is a medicinal plant. Extracts from alkanet roots have applications as natural food coloring agents. In addition, they proven antioxidant effects. Three classical solvents (ethanol and acidified ethanol/water) four eutectic (NADES)-choline chloride/urea; choline chloride/citric acid; chloride/lactic sodium acetate/formic acid-were compared for their effectiveness "green" the extraction of pigment. Notably, this study first to apply chloride-based NADESs extraction, providing comprehensive profile key bioactive compounds contributions activity using UV/Vis FT-IR spectrometry, GC-MS, HPLC-PDA-MS. Among solvents, 50% ethanol showed highest capacity, indicated by its total flavonoid (708 ± 32 mgCE/L) phenolic (1318 63 mgGAE/L) content. However, extract exhibited alkaloid content (256 15 µg/L) other solvents. Consequently, absolute was identified more suitable alternative. NADES, acid (1:2 mol/mol, NADES4) only one show presence alkannins. This also contained high levels phenols (355 21 mg GAE/L) tannins (163 10 CE/L), strong potential (DPPH: 131 µmol TE/g dw, FRAP: 7.49 Fe(II)/mg dw), significantly lower (7.0 0.5 µg/L). Comparative analyses that outperformed those prepared with ethanolic NADES.

Language: Английский

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Formulation and Evaluation of Liposomal Herbal Hair Serum Using Alkanet Root Extract

Published: March 24, 2025

In recent years, hair-related problems have become a significant source of stress and depression for many individuals, primarily driven by factors such as genetics, hormonal changes, environmental influences, the use chemical hair products. This research paper aims in formulation evaluation herbal serum using Alkanna tinctoria root extract, alongside various natural ingredients, to address prevalent problems. Alkanet is ingredient that has potential benefits with rich history traditional use. It alkannin shikonin promotes growth other pharmacological actions. times Herbal more popular due its lesser side effects when compared synthetic More popularly, Hair preferred people over oil light weight non-greasy nature. The developed focus on promoting growth, enhancing manageability, minimizing associated alternatives. incorporates liposomes, which active encapsulated, improve delivery efficacy ingredients. Evaluation conducted through physical appearance, viscosity, skin irritation tests, antimicrobial against Malassezia globosa, demonstrated promising results, indicating formulated safe effective everyday results indicate this offers nourishing approach care, could significantly benefit individuals facing challenges. represents advancement presenting non-toxic alternative conventional

Language: Английский

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Use of Inulin and Pumpkin Oil in the Manufacture of High-Quality Mortadella-Style Sausage from Buffalo Meat

Foods, Journal Year: 2025, Volume and Issue: 14(8), P. 1427 - 1427

Published: April 21, 2025

The growing demand for healthier meat products has driven the reformulation of processed meats to reduce saturated fat while preserving sensory and technological attributes. Buffalo (Bubalus bubalis), with its high protein content, low intramuscular fat, favorable fatty acid profile, offers a promising base formulations. However, content may compromise texture, juiciness, flavor, necessitating strategies optimize product quality. This study investigated effects replacing pork inulin pumpkin seed oil in cooked buffalo product, focusing on compositional, oxidative, microbiological, parameters. Two plant-based ingredients were selected: from chicory, used as mimic due gel-forming ability, oil, structural analog antimicrobial activity. Preliminary trials identified optimal concentrations balancing functional performance. A 2% inclusion exceeding vitro MIC (0.4–1.5%), ensured effectiveness food matrix. Reformulated exhibited significantly reduced (p < 0.05), enhanced fiber, lipid profile rich polyunsaturated acids (>45%), qualifying European Union health claims. Oxidative stability improved 0.01), analysis revealed aroma complexity, nutty roasted notes. Microbiological assessments confirmed protective effect against spoilage bacteria. These results support development nutritionally improved, microbiologically safer such mortadella-style sausage, also offering broader innovation reformulating products.

Language: Английский

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Effect of Nutmeg (Myristica fragrans) and Tea Tree (Melaleuca alternifolia) Essential Oils on the Oxidative and Microbial Stability of Chicken Fillets During Refrigerated Storage

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4139 - 4139

Published: Dec. 20, 2024

The current study investigated the impact of nutmeg essential oil (NEO) and tea tree (TTEO) on preservation raw chicken fillets during nine days refrigerated storage study. primary aim was to explore antioxidant antimicrobial properties these oils (EOs) assess their ability extend shelf life poultry meat. Gas chromatography–mass spectrometry (GC-MS) utilized identify chemical compositions NEO TTEO, revealing presence compounds like myristicin terpenoids, known for activities. Antioxidant were evaluated using DPPH ABTS radical scavenging assays, where both exhibited potent free abilities, with showing higher efficacy than TTEO. EOs showed potential, exhibiting significant antibacterial activities against tested Gram-positive Gram-negative pathogens, such as Staphylococcus aureus Escherichia coli, respectively. Raw treated either or TTEO at 1% analyzed physico-chemical, microbiological, sensory attributes. Results demonstrated that NEO- TTEO-treated samples maintained better microbiological qualities, lower total viable counts enhanced attributes, color odor, compared control samples. Furthermore, effectively delayed spoilage, extending by up seven days. This concludes test’s can be considered natural preservatives enhancing safety quality

Language: Английский

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Effect of Alkanna tinctoria Root Against MRSA and MDR-Pseudomonas aeruginosa Biofilms on Excision Wound in Diabetic Mice: Comparative Study Between Methanolic Extract and Traditional Hydrophobic Preparation

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 991 - 991

Published: Nov. 29, 2024

Alkanna tinctoria, commonly called dyer's alkanet (family-Boraginaceae), is used traditionally in Saudi Arabia to treat skin infections. A methanolic extract and a traditional formulation of the root folklore were prepared. LC-MS analysis was conducted identify probable compounds present hydrophobic formulation. The vivo activity on excision wound evaluated diabetic mice while crystal violet assay employed for vitro evaluation. Human keratinocyte (HaCaT) cells study cytotoxic effects. Several phytoconstituents revealed by formulation, three constituents same. ointment exhibited antibacterial antibiofilm against both tested pathogens. relatively more HaCaT compared did not significantly affect healing, whereas accelerated healing mice. results that A. tinctoria its an effective agent but plant does wounds.

Language: Английский

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