Membrane Localization of RNase Y Is Important for Global Gene Expression in Bacillus subtilis DOI Open Access

Soumaya Laalami,

Marina Cavaiuolo, Jacques Oberto

et al.

Published: July 9, 2024

Abstract: RNase Y is an endoribonuclease that governs global mRNA turnover/processing in Ba-cillus subtilis and likely many other bacteria. The enzyme anchored at the cell membrane, a pseudo-compartmentalization appears coherent with its task to initiate decay of mRNAs translated predominantly periphery. However, reason consequence for attachment membrane are largely unknown. We have studied strain expressing wild-type levels cytoplasmic form from chromosomal locus. This exhibits slow growth phenotype similar null mutant. Genome-wide data show significant effect on expression hundreds genes. Certain RNA substrates clearly require but others do not. found no correlation between stabilization cellular location or function encoded proteins. Y-complex, specificity factor, also recognize which able restore corresponding transcripts. propose required functional interaction coding non-coding RNAs, limiting cleavage certain potentially avoid unfavorable competition ribonucleases like J evolutionarily conserved speci-ficity.

Language: Английский

Membrane Localization of RNase Y Is Important for Global Gene Expression in Bacillus subtilis DOI Open Access

Soumaya Laalami,

Marina Cavaiuolo, Jacques Oberto

et al.

Published: July 9, 2024

Abstract: RNase Y is an endoribonuclease that governs global mRNA turnover/processing in Ba-cillus subtilis and likely many other bacteria. The enzyme anchored at the cell membrane, a pseudo-compartmentalization appears coherent with its task to initiate decay of mRNAs translated predominantly periphery. However, reason consequence for attachment membrane are largely unknown. We have studied strain expressing wild-type levels cytoplasmic form from chromosomal locus. This exhibits slow growth phenotype similar null mutant. Genome-wide data show significant effect on expression hundreds genes. Certain RNA substrates clearly require but others do not. found no correlation between stabilization cellular location or function encoded proteins. Y-complex, specificity factor, also recognize which able restore corresponding transcripts. propose required functional interaction coding non-coding RNAs, limiting cleavage certain potentially avoid unfavorable competition ribonucleases like J evolutionarily conserved speci-ficity.

Language: Английский

Citations

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