Enhanced Pathogenic Consequences Induced by a Seven-Amino-Acid Extension in the G Protein of the HRSV BA9 Genotype
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(5), P. 2081 - 2081
Published: Feb. 27, 2025
In
a
previous
outbreak
of
the
human
respiratory
syncytial
virus
(HRSV),
we
identified
variant
strain
genotype
BA9
with
seven-amino-acid
extension
(Q-R-L-Q-S-Y-A)
at
C-terminus
attachment
protein
(G).
To
assess
impact
this
on
virulence
HRSV,
two
full-length
infectious
clones
using
wild
as
backbone,
one
containing
(rRSV
WT),
and
other
deleting
Δ7AA),
were
successfully
rescued
reverse
genetics
system.
The
biological
properties
viruses
then
compared
analyzed
in
vitro
vivo.
Compared
to
rRSV
Δ7AA,
WT
exhibited
larger
plaque
size
more
pronounced
suppression
host
cell
innate
immune
response
(IFN-β
levels:
154.33
pg/mL
vs.
11.27
pg/mL).
demonstrated
increased
adaptability
mice,
10-fold
higher
lung
viral
load
stronger
inflammatory
following
intranasal
exposure.
Our
study
primarily
that
C-terminal
G
HRSV
can
enhance
virulence,
underscoring
importance
virological
surveillance
prevention
treatment
severe
HRSV-related
disease.
Language: Английский
The Role of the CX3CR1-CX3CL1 Axis in Respiratory Syncytial Virus Infection and the Triggered Immune Response
Published: July 11, 2024
Respiratory
Syncytial
Virus
is
a
common
respiratory
pathogen
known
for
causing
illnesses,
ranging
from
mild
symptoms
to
severe
lower
tract
infections
in
infants
and
the
elderly.
This
virus
responsible
one-third
of
pneumonia
deaths
pediatric
population,
but
there
are
currently
only
few
effective
vaccines
pharmacological
treatments.
The
frac-talkine
(CX3CL1)
receptor
(CX3CR1)
co-receptor
RSV
expressed
by
airway
epithelial
cells
diversity
immune
cells.
Recent
research
has
highlighted
role
CX3CR1
host
response
infection,
its
potential
development
an
efficient
vaccine
novel
Language: Английский