Zika Virus in Extracellular Vesicles: Insights from Integrated Proteomic and Metabolomic Dependent Regulation of B Cell and PI3K/AKT/mTOR Signaling Pathway DOI Open Access
Letícia Gomes de Pontes, Lucila Akune Barreiros, Lillian Nunes Gomes

et al.

Published: Aug. 12, 2024

Background; Cell-released extracellular vesicles (EVs) acting as 'metabolically and proteolytically active machines,' show potential in metabolomic proteomic analysis of serum EVs. Despite diverse challenges, post-isolation omics characterization EVs offers crucial insights for effective analysis; (2) Methods: The research, involved children with Congenital Zika Syndrome, utilizing mass spectrometry proteomics GC-MS metabolite identification. Vesicles were isolated using Izon qEV columns, quantified, characterized by NTA TEM. Data employed Cytoescape/String MetaboAnalyst, revealing variations metabolic profiles among groups through PCA volcano plots. Proteins Metabolite set enrichment provided biologically meaningful patterns to enriched metabolites; (3) Results: Using molecular exclusion chromatography, the characterized, size variations. Protein identified 13 significantly altered proteins, including upregulated (e.g., AOM8Q6 - IGLC7) downregulated Q8TD86 CALML6) ones. indicated involvement PI3K-AKT-mTOR pathway suggested a role Angiotensin inhibition CZS+. Upstream mTOR, Akt is central signaling molecule PI3K plays critical roles brain development well synaptic plasticity important Virus. study provides into mechanisms associated CZS; (4) Conclusions: pinpointed valuable possible biomarkers, specifically proteins metabolites, virus (ZIKV) infection. It stresses necessity broader investigations advanced techniques uncover targets, potentially advancing pharmacological strategies.

Language: Английский

Zika Virus in Extracellular Vesicles: Insights from Integrated Proteomic and Metabolomic Dependent Regulation of B Cell and PI3K/AKT/mTOR Signaling Pathway DOI Open Access
Letícia Gomes de Pontes, Lucila Akune Barreiros, Lillian Nunes Gomes

et al.

Published: Aug. 12, 2024

Background; Cell-released extracellular vesicles (EVs) acting as 'metabolically and proteolytically active machines,' show potential in metabolomic proteomic analysis of serum EVs. Despite diverse challenges, post-isolation omics characterization EVs offers crucial insights for effective analysis; (2) Methods: The research, involved children with Congenital Zika Syndrome, utilizing mass spectrometry proteomics GC-MS metabolite identification. Vesicles were isolated using Izon qEV columns, quantified, characterized by NTA TEM. Data employed Cytoescape/String MetaboAnalyst, revealing variations metabolic profiles among groups through PCA volcano plots. Proteins Metabolite set enrichment provided biologically meaningful patterns to enriched metabolites; (3) Results: Using molecular exclusion chromatography, the characterized, size variations. Protein identified 13 significantly altered proteins, including upregulated (e.g., AOM8Q6 - IGLC7) downregulated Q8TD86 CALML6) ones. indicated involvement PI3K-AKT-mTOR pathway suggested a role Angiotensin inhibition CZS+. Upstream mTOR, Akt is central signaling molecule PI3K plays critical roles brain development well synaptic plasticity important Virus. study provides into mechanisms associated CZS; (4) Conclusions: pinpointed valuable possible biomarkers, specifically proteins metabolites, virus (ZIKV) infection. It stresses necessity broader investigations advanced techniques uncover targets, potentially advancing pharmacological strategies.

Language: Английский

Citations

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