pH-Dependent Extraction of Antioxidant Peptides from Red Seaweed: A Sequential Approach DOI Open Access
Sakhi Ghelichi, Ann‐Dorit Moltke Sørensen, Grazielle Náthia-Neves

et al.

Published: Aug. 22, 2024

The present study adopted a diverse approach to obtain antioxidant peptides from red seaweed, starting with an aqueous extraction of the entire seaweed followed by enzymatic hydrolysis solid residues first step. Additionally, impact three different pH levels (i.e. 3, 6, and 9) during phase was examined for their influence on outcomes. findings revealed that fraction resulting sequential process contained significantly higher proteins amino acids compared other fractions (p < 0.05). Furthermore, (IC50 ranging 2.29 8.15) proved be more effective free radical scavengers than liquid either 9.03 10.41 or not obtained at highest concentration tested) both stages Among fractions, those produced under alkaline conditions did exhibit as potent scavenging abilities acidic nearly neutral conditions. most metal ion chelating activity found in derived subsequent stage, particularly 3 = 0.63 ± 0.04) 6 0.89 0.07), which were initial stage Given no significant difference noted total phenolic content these corresponding (3.79 0.05 vs. 3.48 0.02 2.43 0.22 2.51 0.00 6) > 0.05), demonstrated properties might attributed potential roles bioactive such histidine, glutamic acid, aspartic tyrosine, methionine, whether integrated within structure peptides.

Language: Английский

Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels DOI Creative Commons
Sakhi Ghelichi,

Mona Hajfathalian,

Sara Falcione

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(1), P. 35 - 35

Published: Jan. 12, 2025

This research examined antioxidant and anti-obesity effects of Palmaria palmata extracts obtained through acidic or alkaline treatments subsequent pH adjustments. After two rounds extraction, the were separated from biomass adjusted to different values: for extracts, 3 (no adjustment), 6, 9, 12; 12 3. The findings revealed that extraction medium as well adjustments significantly influenced composition in terms protein content recovery, amino acids, phenolic compounds (p < 0.05). Acidic conditions produced with potent radical scavenging, especially at 6 (IC50 = 0.30 ± 0.04 mg.mL−1), while favored metal chelating, highest Fe2+ chelation 0.65 0.03 mg.mL−1). Moreover, showed inhibitory activities against porcine pancreatic lipase α-amylase, extract 9 showing best properties 5.38 0.34 mg.mL−1 IC50 5.79 α-amylase). However, α-amylase activity was 3.05 0.66 In conclusion, adjusting seaweed notably influences their bioactive properties, likely due changes reactivity interactions such peptides, carbohydrates, polyphenols.

Language: Английский

Citations

0
pH-Dependent Extraction of Antioxidant Peptides from Red Seaweed: A Sequential Approach DOI Open Access
Sakhi Ghelichi, Ann‐Dorit Moltke Sørensen, Grazielle Náthia-Neves

et al.

Published: Aug. 22, 2024

The present study adopted a diverse approach to obtain antioxidant peptides from red seaweed, starting with an aqueous extraction of the entire seaweed followed by enzymatic hydrolysis solid residues first step. Additionally, impact three different pH levels (i.e. 3, 6, and 9) during phase was examined for their influence on outcomes. findings revealed that fraction resulting sequential process contained significantly higher proteins amino acids compared other fractions (p &lt; 0.05). Furthermore, (IC50 ranging 2.29 8.15) proved be more effective free radical scavengers than liquid either 9.03 10.41 or not obtained at highest concentration tested) both stages Among fractions, those produced under alkaline conditions did exhibit as potent scavenging abilities acidic nearly neutral conditions. most metal ion chelating activity found in derived subsequent stage, particularly 3 = 0.63 ± 0.04) 6 0.89 0.07), which were initial stage Given no significant difference noted total phenolic content these corresponding (3.79 0.05 vs. 3.48 0.02 2.43 0.22 2.51 0.00 6) &gt; 0.05), demonstrated properties might attributed potential roles bioactive such histidine, glutamic acid, aspartic tyrosine, methionine, whether integrated within structure peptides.

Language: Английский

Citations

2