Morphometric Differences in Sperm Subpopulations in Frozen-Thawed Semen of Two Bovine Subspecies DOI Open Access
Francisco Sevilla, Ignacio Araya‐Zúñiga, Miguel Á. Silvestre

et al.

Published: Aug. 22, 2024

[Objetive] The objective of the present study was to characterize different sperm subpopulations based on morphometric parameters frozen-thawed semen in two bovine subspecies using a CASA system. [Methodology] experiment carried out at Costa Rica Institute Technology from May December 2023. Spermatozoa 10 bulls (five animals each species Bos taurus and indicus) were evaluated after thawing doses by an ISAS®v1, computer-assisted analysis (CASA)-Morph Sub-populations head spermatozoa characterized multivariate procedures such as principal components (PCs) clustering methods (k-means model) [Results] ejaculate male exhibits significant heterogeneity comprises diverse with differing patterns. Three identified three PCs: t size, shape, symmetry degree which it pyriform. proportions varied two-bovine subspecies; indicus. Results indicated that SP1, SP2, SP3 for PC criteria these differences relevant. variability morphometry assessments underscores need standardize evaluation protocols [Conclusions] These findings highlight importance knowing diversity between both improve vitro tests distinguish subspecies.

Language: Английский

Morphometric Differences in Sperm Subpopulations in Frozen-Thawed Semen of Two Bovine Subspecies DOI Open Access
Francisco Sevilla, Ignacio Araya‐Zúñiga, Miguel Á. Silvestre

et al.

Published: Aug. 22, 2024

[Objetive] The objective of the present study was to characterize different sperm subpopulations based on morphometric parameters frozen-thawed semen in two bovine subspecies using a CASA system. [Methodology] experiment carried out at Costa Rica Institute Technology from May December 2023. Spermatozoa 10 bulls (five animals each species Bos taurus and indicus) were evaluated after thawing doses by an ISAS®v1, computer-assisted analysis (CASA)-Morph Sub-populations head spermatozoa characterized multivariate procedures such as principal components (PCs) clustering methods (k-means model) [Results] ejaculate male exhibits significant heterogeneity comprises diverse with differing patterns. Three identified three PCs: t size, shape, symmetry degree which it pyriform. proportions varied two-bovine subspecies; indicus. Results indicated that SP1, SP2, SP3 for PC criteria these differences relevant. variability morphometry assessments underscores need standardize evaluation protocols [Conclusions] These findings highlight importance knowing diversity between both improve vitro tests distinguish subspecies.

Language: Английский

Citations

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