Transcytosis of T4 Bacteriophage Through Intestinal Cells Enhances Its Immune Activation
Viruses,
Journal Year:
2025,
Volume and Issue:
17(1), P. 134 - 134
Published: Jan. 19, 2025
Interactions
between
bacteriophages
with
mammalian
immune
cells
are
of
great
interest
and
most
phages
possess
at
least
one
molecular
pattern
(nucleic
acid,
sugar
residue,
or
protein
structure)
that
is
recognizable
to
the
system
through
pathogen
associated
(PAMP)
receptors
(i.e.,
TLRs).
Given
reside
in
same
body
niches
as
bacteria,
they
share
propensity
stimulate
quench
responses
depending
on
nature
their
interactions
host
cells.
While
vitro
research
focuses
outcomes
direct
application
interest,
potential
impact
transcytosis
intestinal
barrier
has
yet
be
considered.
As
a
necessary
step
healthy
systems
for
access
by
phage
underlying
cell
populations,
it
imperative
understand
how
this
may
play
role
activation.
We
compared
activation
macrophages
(as
measured
TNFα
secretion)
following
those
stimulated
incubation
transcytosed
polarized
Caco2
epithelial
model.
Our
results
demonstrate
capable
activating
secretion
upon
contact
maintain
stimulatory
capability
transcytosis.
Furthermore,
enhanced
occurring
an
equivalent
multiplicity
directly
applied
phage.
Language: Английский
Comprehensive Review on Phage Therapy and Phage-Based Drug Development
Published: Aug. 27, 2024
Phage
therapy,
the
use
of
bacteriophages
(phages)
to
treat
bacterial
infections,
is
regaining
momentum
as
a
promising
weapon
against
rising
threat
multidrug-resistant
(MDR)
bacteria.
This
comprehensive
review
explores
historical
context,
modern
resurgence
phage
and
phage-facilitated
advancements
in
medical
technological
fields.
It
details
mechanisms
action
applications
phages
treating
MDR
particularly
those
associated
with
biofilms
intracellular
pathogens.
The
further
highlights
innovative
uses
vaccine
development,
cancer
gene
delivery
vectors.
Despite
its
targeted
efficient
approach,
therapy
faces
challenges
related
stability,
immune
response,
regulatory
approval.
By
examining
these
areas
detail,
this
underscores
immense
potential
remaining
hurdles
integrating
phage-based
therapies
into
practices.
Language: Английский
Phage cocktail amikacin combination as a potential therapy for bacteremia associated with carbapenemase producing colistin resistant Klebsiella pneumoniae
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 22, 2024
The
increasing
occurrence
of
hospital-associated
infections,
particularly
bacteremia,
caused
by
extensively
drug-resistant
(XDR)
carbapenemase-producing
colistin-resistant
Klebsiella
pneumoniae
highlights
a
critical
requirement
to
discover
new
therapeutic
alternatives.
Bacteriophages
having
host-specific
bacteriolytic
effects
are
promising
alternatives
for
combating
these
pathogens.
Among
12
phages
isolated
from
public
wastewater
in
Thailand,
two
phages-vB_kpnM_05
(myovirus)
and
vB_kpnP_08
(podovirus)
showed
broad-host
range,
producing
activities
against
81.3%
(n
=
26)
78.1%
25)
32
XDR
K.
pneumoniae,
with
capsular
types—K15,
K17,
K50,
K51,
K52/wzi-50
K2/wzi-2.
Both
short
replication
times,
large
burst
sizes
rapid
adsorptions.
They
exhibited
significant
stability
under
various
environmental
conditions.
Genomic
analysis
revealed
that
both
genetically
distinct
Myoviridae
Podoviridae
family,
the
lack
toxin,
virulence,
lysogeny
antibiotic
resistance
genes.
These
characteristics
highlighted
their
potential
utilizing
phage
therapy
pneumoniae.
Although
cocktail
combining
vB_kpnM_05
provided
bacteriolysis
longer
duration
(8
h)
than
its
monophage
(6
h),
bacterial
regrowth
was
observed
which
suggested
an
evitable
development
phages'
selection
pressures.
Future
study
will
be
undertaken
elucidate
precise
mechanisms
developed
associated
fitness
cost.
Remarkably,
amikacin
at
sub-inhibitory
concentrations
produced
potent
synergy
completely
suppressing
vitro.
Our
demonstrated
prophylactic
effectiveness
cocktail-amikacin
combination
as
alternative
strategy
overcoming
bacteremia
carbapenemase
colistin
vivo.
Language: Английский