The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity through the cAMP/Rac1 Pathway DOI Open Access
Tingting Gao,

Ruiyuan Gu,

Heng Wang

et al.

Published: Aug. 27, 2024

Background: Contrast-induced acute kidney injury (CIAKI) is a common complication in hospital-acquired renal conditions, yet its pathogenesis remains unclear and effective treatments are limited. Intermedin (IMD), peptide related to the calcitonin gene, exhibits vasodilatory endothelial barrier-stabilizing effects via receptor-like receptor (CLR). However, potential of IMD mitigating CIAKI has not been thoroughly investigated. Aims: This study aimed delineate protective role through both vitro vivo models, with particular focus on elucidating underlying mechanisms, specifically cAMP/Rac1 signaling pathway. Methods: Human umbilical vein cells (HUVECs) were treated iohexol simulate vitro. The evaluated using CCK8 assay, flow cytometry, ELISA, western blotting. A rat model was developed assess peritubular capillary cell function range techniques, including histopathology, immunohistochemistry, immunofluorescence, blotting, transmission electron microscopy. Results: In vitro, significantly enhanced HUVEC viability mitigated iohexol-induced toxicity by preserving intercellular adhesion junctions activating attenuated Rac1 inhibition. vivo, caused considerable damage junctions, leading impaired function. treatment markedly improved function, an effect that abolished Conclusion: protects against pathway, thereby integrity induced contrast media. These findings underscore therapeutic emphasize critical pathway protection.

Language: Английский

The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity through the cAMP/Rac1 Pathway DOI Open Access
Tingting Gao,

Ruiyuan Gu,

Heng Wang

et al.

Published: Aug. 27, 2024

Background: Contrast-induced acute kidney injury (CIAKI) is a common complication in hospital-acquired renal conditions, yet its pathogenesis remains unclear and effective treatments are limited. Intermedin (IMD), peptide related to the calcitonin gene, exhibits vasodilatory endothelial barrier-stabilizing effects via receptor-like receptor (CLR). However, potential of IMD mitigating CIAKI has not been thoroughly investigated. Aims: This study aimed delineate protective role through both vitro vivo models, with particular focus on elucidating underlying mechanisms, specifically cAMP/Rac1 signaling pathway. Methods: Human umbilical vein cells (HUVECs) were treated iohexol simulate vitro. The evaluated using CCK8 assay, flow cytometry, ELISA, western blotting. A rat model was developed assess peritubular capillary cell function range techniques, including histopathology, immunohistochemistry, immunofluorescence, blotting, transmission electron microscopy. Results: In vitro, significantly enhanced HUVEC viability mitigated iohexol-induced toxicity by preserving intercellular adhesion junctions activating attenuated Rac1 inhibition. vivo, caused considerable damage junctions, leading impaired function. treatment markedly improved function, an effect that abolished Conclusion: protects against pathway, thereby integrity induced contrast media. These findings underscore therapeutic emphasize critical pathway protection.

Language: Английский

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