Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways DOI Open Access
Matthew Zakaria,

Justin Matta,

Yazan Honjol

et al.

Published: Aug. 29, 2024

Applying cold to a bone injury can aid healing, though its mechanisms are complex. This study investigates how therapy impacts repair optimize healing. Cold was applied rodent model, with physiological responses analyzed. Vasoconstriction mediated by an increase in TRP channels, TRPA1 (p=0.012) and TRPM8 (p<0.001), within cortical defects enhancing sensory response blood flow regulation. exposure also elevated hypoxia (p < 0.01) VEGF expression 0.001), promoting angiogenesis vital for regeneration. Increased of osteogenic proteins PGC-1α = 0.039) RBM3 (p<0.008) suggests stimulated reparative processes. Enhanced osteoblast differentiation ALP presence at days 5 (three-fold, p=0.014) 10 (two-fold, p=0.010) were observed, along increased osteocalcin (OCN) day p 0.010) indicating mature osteoblasts capable mineralization. These findings highlight therapy's multifaceted effects on repair, offering insights therapeutic strategies.

Language: Английский

Role of TRP Channels in Cancer-Induced Bone Pain DOI Open Access
Flaminia Coluzzi, Maria Sole Scerpa, Elisa Alessandri

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1229 - 1229

Published: Jan. 30, 2025

The burden of cancer is growing in almost every country. Bone metastases significantly affect the prognosis and lead to an increase mortality morbidity. management cancer-induced bone pain (CIBP) still shows various unmet needs. Opioid use burdened by a number possible side effects. Moreover, recent progresses treatment increased life expectancy patients, even those with metastatic disease. In this narrative review, we reported main findings regarding TRP channel function models. cation channels play key role different functions cells, including regulation their potential for metastasization, are involved pathways perception, through peripheral central Genetic deletion decreased sensitivity following tumour cell inoculation. Preclinical data suggest modulators some channels, such as TRPV1, TRPA1, TRPM7 TRPM8. Clinical results scarce; however, physiological modulating remodelling involvement preclinical models have garnered interest areas research last few years, innovative analgesic strategies that may overcome long-term effects opioids.

Language: Английский

Citations

0
Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways DOI Open Access
Matthew Zakaria,

Justin Matta,

Yazan Honjol

et al.

Published: Aug. 29, 2024

Applying cold to a bone injury can aid healing, though its mechanisms are complex. This study investigates how therapy impacts repair optimize healing. Cold was applied rodent model, with physiological responses analyzed. Vasoconstriction mediated by an increase in TRP channels, TRPA1 (p=0.012) and TRPM8 (p<0.001), within cortical defects enhancing sensory response blood flow regulation. exposure also elevated hypoxia (p < 0.01) VEGF expression 0.001), promoting angiogenesis vital for regeneration. Increased of osteogenic proteins PGC-1α = 0.039) RBM3 (p<0.008) suggests stimulated reparative processes. Enhanced osteoblast differentiation ALP presence at days 5 (three-fold, p=0.014) 10 (two-fold, p=0.010) were observed, along increased osteocalcin (OCN) day p 0.010) indicating mature osteoblasts capable mineralization. These findings highlight therapy's multifaceted effects on repair, offering insights therapeutic strategies.

Language: Английский

Citations

2