Diversity of Neurotransmitter Producing Human Skin Commensals DOI Open Access
Samane Rahmdel,

Moushumi Purkayastha,

Mulugeta Nega

et al.

Published: Oct. 24, 2024

Recent findings indicate that human microbiota can excrete trace amines, dopamine, and serotonin. These neurotransmitters (NTs) either affect classical neurotransmitter signaling or directly trigger amine-associated receptors (TAARs), with still unclear consequences for host physiology. Compared to gut microbiota, less information is available on the role of skin in NT production. To explore this, 1909 isolates, mainly from genera Staphylococcus, Bacillus, Corynebacterium, were tested Only 6.7% isolates capable producing NTs, all which belonged Staphylococcus genus. Based substrate specificity, we identified two distinct profiles among producers. One group primarily produced tryptamine (TRY) phenylethylamine (PEA), while other tyramine (TYM) dopamine (Dopa). differing production could be attributed activity aromatic amino acid decarboxylase enzymes, SadA TDC, responsible generating TRY/PEA TYM/Dopa product spectra, respectively. TDC orthologues differ structure size; has approximately 475 acids, whereas TDC-type consists about 620 acids. The genomic localization respective genes also varies: tdc are typically found small, conserved gene clusters, sadA not. Heterologous expression Escherichia coli yielded same spectrum as parent strains. possible effects microbiota-derived NTs neuroreceptor remain investigated.

Language: Английский

Diversity of Neurotransmitter Producing Human Skin Commensals DOI Open Access
Samane Rahmdel,

Moushumi Purkayastha,

Mulugeta Nega

et al.

Published: Oct. 24, 2024

Recent findings indicate that human microbiota can excrete trace amines, dopamine, and serotonin. These neurotransmitters (NTs) either affect classical neurotransmitter signaling or directly trigger amine-associated receptors (TAARs), with still unclear consequences for host physiology. Compared to gut microbiota, less information is available on the role of skin in NT production. To explore this, 1909 isolates, mainly from genera Staphylococcus, Bacillus, Corynebacterium, were tested Only 6.7% isolates capable producing NTs, all which belonged Staphylococcus genus. Based substrate specificity, we identified two distinct profiles among producers. One group primarily produced tryptamine (TRY) phenylethylamine (PEA), while other tyramine (TYM) dopamine (Dopa). differing production could be attributed activity aromatic amino acid decarboxylase enzymes, SadA TDC, responsible generating TRY/PEA TYM/Dopa product spectra, respectively. TDC orthologues differ structure size; has approximately 475 acids, whereas TDC-type consists about 620 acids. The genomic localization respective genes also varies: tdc are typically found small, conserved gene clusters, sadA not. Heterologous expression Escherichia coli yielded same spectrum as parent strains. possible effects microbiota-derived NTs neuroreceptor remain investigated.

Language: Английский

Citations

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