Evaluation of the Pro-Angiogenic Effect in Chicken Embryo Chorioallantoic Membrane (CAM) Model and the In Vivo Wound Healing Potential of the Moroccan Polysaccharide-Rich Algae Osmundea pinnatifida DOI Open Access

Bengaly Zakaria,

Malek Eleroui, Ben Saad Hajer

et al.

Published: Oct. 23, 2024

Various methods have been studied to speed up the wound healing process. Promoting rapid development of blood vessels, also known as angiogenesis, is essential this significantly accelerates skin regeneration. This study identified a polysaccharide from red alga Osmundea pinnatifida (PSOP) that promotes angiogenesis and healing. Firstly, we analyzed structural characteristics PSOP using various techniques such UV-visible spectrum, Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (X-RD), scanning electron microscopy (SEM), high-performance liquid chromatography coupled refractive index detector (HPLC-RID). We evaluated antioxidant properties in vitro tests for DPPH free radical scavenging capacity, iron reduction capacity (RPA), total (TAC). Additionally, observed significant increase new vessel formation chorioallantoic membrane model (CAM assay) fertilized hen egg, indicating pro-angiogenic effect PSOP. Furthermore, tested wound-healing potential hydrogel vivo excision wounds Wistar rat model. The results showed notable acceleration closure an improvement tissue regeneration collagen deposition quality within 11 days, strong activity Our computational suggested units bind cyclooxygenase 2 (COX-2) vascular endothelial growth factor (VEGF) with acceptable affinities interactions, which might explain beneficial seen experimental assays. findings support treatment due its potential.

Language: Английский

Evaluation of the Pro-Angiogenic Effect in Chicken Embryo Chorioallantoic Membrane (CAM) Model and the In Vivo Wound Healing Potential of the Moroccan Polysaccharide-Rich Algae Osmundea pinnatifida DOI Open Access

Bengaly Zakaria,

Malek Eleroui, Ben Saad Hajer

et al.

Published: Oct. 23, 2024

Various methods have been studied to speed up the wound healing process. Promoting rapid development of blood vessels, also known as angiogenesis, is essential this significantly accelerates skin regeneration. This study identified a polysaccharide from red alga Osmundea pinnatifida (PSOP) that promotes angiogenesis and healing. Firstly, we analyzed structural characteristics PSOP using various techniques such UV-visible spectrum, Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (X-RD), scanning electron microscopy (SEM), high-performance liquid chromatography coupled refractive index detector (HPLC-RID). We evaluated antioxidant properties in vitro tests for DPPH free radical scavenging capacity, iron reduction capacity (RPA), total (TAC). Additionally, observed significant increase new vessel formation chorioallantoic membrane model (CAM assay) fertilized hen egg, indicating pro-angiogenic effect PSOP. Furthermore, tested wound-healing potential hydrogel vivo excision wounds Wistar rat model. The results showed notable acceleration closure an improvement tissue regeneration collagen deposition quality within 11 days, strong activity Our computational suggested units bind cyclooxygenase 2 (COX-2) vascular endothelial growth factor (VEGF) with acceptable affinities interactions, which might explain beneficial seen experimental assays. findings support treatment due its potential.

Language: Английский

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