Spatiotemporal modeling reveals high-resolution invasion states in glioblastoma DOI Creative Commons
Varsha Thoppey Manoharan, Aly Abdelkareem, Samuel M. Brown

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 7, 2023

Abstract Diffuse invasion of glioblastoma cells through normal brain tissue is a key contributor to tumor aggressiveness, resistance conventional therapies, and dismal prognosis in patients. A deeper understanding how components the microenvironment (TME) contribute overall organization programs may reveal opportunities for improved therapeutic strategies. Towards this goal, we applied novel computational workflow spatiotemporally profiled GBM xenograft cohort, leveraging ability distinguish human from mouse TME overcome previous limitations analysis diffuse invasion. Our analytic approach, based on unsupervised deconvolution, performs reference-free discovery cell types activities within complete ecosystem. We present comprehensive catalogue 15 set spatiotemporal context 90 types, activities, anatomic structures. Distinct related were aligned with routes perivascular, white matter, parenchymal Furthermore, sub-modules genes serving as program network hubs highly prognostic The compendium presented here provides basis rational targeting and/or components. anticipate that our approach will facilitate an ecosystem-level immediate long-term consequences such perturbations, including identification compensatory inform combinatorial therapies.

Language: Английский

Spatiotemporal modeling reveals high-resolution invasion states in glioblastoma DOI Creative Commons
Varsha Thoppey Manoharan, Aly Abdelkareem,

Gurveer Gill

et al.

Genome biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 10, 2024

Diffuse invasion of glioblastoma cells through normal brain tissue is a key contributor to tumor aggressiveness, resistance conventional therapies, and dismal prognosis in patients. A deeper understanding how components the microenvironment (TME) contribute overall organization programs may reveal opportunities for improved therapeutic strategies.

Language: Английский

Citations

1
Spatiotemporal modeling reveals high-resolution invasion states in glioblastoma DOI Creative Commons
Varsha Thoppey Manoharan, Aly Abdelkareem, Samuel M. Brown

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 7, 2023

Abstract Diffuse invasion of glioblastoma cells through normal brain tissue is a key contributor to tumor aggressiveness, resistance conventional therapies, and dismal prognosis in patients. A deeper understanding how components the microenvironment (TME) contribute overall organization programs may reveal opportunities for improved therapeutic strategies. Towards this goal, we applied novel computational workflow spatiotemporally profiled GBM xenograft cohort, leveraging ability distinguish human from mouse TME overcome previous limitations analysis diffuse invasion. Our analytic approach, based on unsupervised deconvolution, performs reference-free discovery cell types activities within complete ecosystem. We present comprehensive catalogue 15 set spatiotemporal context 90 types, activities, anatomic structures. Distinct related were aligned with routes perivascular, white matter, parenchymal Furthermore, sub-modules genes serving as program network hubs highly prognostic The compendium presented here provides basis rational targeting and/or components. anticipate that our approach will facilitate an ecosystem-level immediate long-term consequences such perturbations, including identification compensatory inform combinatorial therapies.

Language: Английский

Citations

0