Enhanced systemic antitumor efficacy of PD‐1/PD‐L1 blockade with immunological response induced by photodynamic therapy DOI Creative Commons
Takumi Sonokawa, Yukio Fujiwara, Cheng Pan

et al.

Thoracic Cancer, Journal Year: 2024, Volume and Issue: 15(18), P. 1429 - 1436

Published: May 13, 2024

Abstract Background Photodynamic therapy (PDT) is an antitumor and has traditionally been regarded as a localized in itself. However, recent reports have shown that it not only exerts direct cytotoxic effect on cancer cells but also enhances body's tumor immunity. We hypothesized the immunological response induced by PDT could potentially enhance efficacy of programmed death‐1 (PD‐1) / death‐ligand 1 (PD‐L1) blockade. Methods The effects colon 26 were investigated vitro using WST assay. whether anti‐PD‐1 antibodies be amplified addition PDT. performed combination randomly allocating tumor‐bearing mice to four treatment groups: control, antibodies, PDT, To analyze microenvironment after treatment, tumors resected pathologically evaluated. Results viability rate decreased proportionally with laser dose. In vivo experiments for combined antibody showed enhanced compared control. Immunohistochemical findings 10 days indicated number CD8+ cells, area Iba‐1+ expressing PD‐L1 significantly higher treated than alone, or Conclusions increased immune cell infiltration into microenvironment. may PD‐1/PD‐L1

Language: Английский

Timing Anti-PD-L1 Checkpoint Blockade Immunotherapy to Enhance Tumor Irradiation DOI Open Access
Seung Young Lee,

Joanna Pagacz,

Sera Averbek

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(3), P. 391 - 391

Published: Jan. 24, 2025

Background: The ability of radiotherapy (RT) to drive anti-tumor immunity is limited by adaptive resistance. While RT induces inflammation and recruits activated tumor-infiltrating lymphocytes (TILs), including cytotoxic T (CTLs), the resulting radiation- IFNγ-dependent PD-L1 expression restores an immunosuppressed tumor microenvironment. Unleashing effective response may require precise sequencing checkpoint blockade immunotherapy (CBI) block signaling before it can mediate its suppressive effects. Methods: Flank tumors formed in BALB/c mice with syngeneic CT26 colon or 4T1 mammary carcinoma cells were treated otherwise ineffective doses ionizing radiation (10 Gy) followed CBI (0.2 mg anti-PD-L1, i.v.) after 0, 1, 3, 5, 7 days, comparing response. Anti-PD-L1 delivery was measured fluorescence, TILs flow cytometry immunofluorescence, immunohistochemistry, size calipers. Results: In both tumors, 10 Gy alone resulted a transient growth delay associated infiltrating CTLs peaking at 3 days 5 days. returned baseline consistent failed potentiate except when injected Gy, which prevented CTL depletion led elimination. Potentially contributing compound effects, anti-PD-L1 penetrated bound more efficiently irradiation. Conclusions: Optimal timing exploit radiation-induced permeability enhance interrupt resistance blocking as peaks offer general strategy external beam protecting potentiating immune

Language: Английский

Citations

0
The cGAS‐STING‐related signature affects the prognosis of colorectal cancer through its regulation of multiple immune cells DOI Creative Commons
Yunlong Li,

Xunliang Jiang,

Hui Cao

et al.

IUBMB Life, Journal Year: 2025, Volume and Issue: 77(3)

Published: March 1, 2025

The cGAS-STING signaling pathway has emerged as a critical player in the immune response against cancer, including colorectal adenocarcinoma (COAD). Understanding impact of this on COAD at multiple omics levels is crucial for advancing cancer immunotherapy and precision medicine. This study aimed to investigate relationship between cGAS-STING-related genes COAD, analyzing gene mutations, copy number variations, DNA methylation, expression uncover pathway's influence prognosis. Utilizing multi-omics sequencing data from TCGA GEO databases, key core were identified further validated through PCR Western blot analysis. Mutations variations CASP8 RIPK1 genes, differential methylation patterns, mRNA specific assessed determine their Validation tissue samples highlighted NLRC3, CASP1, AIM2, CXCL10 pathway. Our findings demonstrate that mutations RIPK1, altered significantly prognosis COAD. identification pathway, particularly CXCL10, led development prognostic model predicting poor tumor outcomes cell infiltration. provides valuable insights into mechanisms offers potential directions future research

Language: Английский

Citations

0
Cellular polarity pilots breast cancer progression and immunosuppression DOI Creative Commons
Jie Huang, Shufeng Luo, Juan Shen

et al.

Oncogene, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Abstract Disrupted cellular polarity (DCP) is a hallmark of solid cancer, the malignant disease epithelial tissues, which occupies ~90% all human cancers. DCP has been identified to affect not only cancer cell’s aggressive behavior but also migration and infiltration immune cells, although precise mechanism DCP-affected tumor-immune cell interaction remains unclear. This review discusses immunosuppressive tumor microenvironments (TME) caused by DCP-driven proliferation with DCP-impaired functions. We will revisit fundamental roles (CP) proteins in sustaining mammary luminal homeostasis, transformation, breast progression. Then, current data on CP involvement activation, maturation, migration, are evaluated. The status effector cells their targeted highlighted regulation, including antigen presentation formation synapses (IS). CP-regulated delivery IS between especially effectors be addressed. Alterations infiltrated or both discussed these aspects. conclude that CP-mediated aggressiveness coupled disability may decide degree responsiveness checkpoint blockade (ICB). Further elucidating dynamics CP- DCP-mediated regulation TME provide more critical insights into dynamics, required invent effective approaches for immunotherapy.

Language: Английский

Citations

0
The crosstalk between lung adenocarcinoma cells and M2 macrophages promotes cancer cell development via the SFRS1/miR-708-5p/PD-L1 axis DOI
Li Xu, Kang Li, Jia Li

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123599 - 123599

Published: April 1, 2025

Language: Английский

Citations

0
Advanced Nanobiomaterial-Mediated Radio-Immunotherapy in Malignant Cancer DOI

Ning Han,

Jun Hu,

Liu‐Gen Li

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Immunotherapy-Boosted Stereotactic Ablative Radiotherapy in Inoperable Early-Stage Non-Small Cell Lung Cancer DOI
Jie Lian, Yi Liu, Shuling Zhang

et al.

Current Treatment Options in Oncology, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

Citations

0
Dietary enrichment with n-3 lc-PUFA-rich fish oil improves preclinical outcome of radiotherapy and anti-PD-L1 combination treatment DOI Creative Commons

Annemarie J. F. Westheim,

Ludwig J. Dubois, Èlia Prades-Sagarra

et al.

Cancer Treatment and Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 100943 - 100943

Published: May 1, 2025

Language: Английский

Citations

0
Chemotherapy elevates cell surface PD-L1 and MHC-I expression in apoptotic gastric cancer cells DOI Creative Commons

Y. Lou,

X Liu,

Chih-Cheng Cheng

et al.

International Journal of Immunopathology and Pharmacology, Journal Year: 2025, Volume and Issue: 39

Published: March 1, 2025

The programmed cell death-ligand 1 (PD-L1) combined positive score is used as a patient selection tool and predictive factor for anti-programmed death-1 (PD-1)/PD-L1 therapy in gastric cancer. However, the expression of PD-L1 major histocompatibility complex class I (MHC-I) can be affected by conventional treatment approaches. In this study, we examined effects chemotherapy on surface MHC-I living apoptotic cancer cells. AGS (moderately differentiated) SNU-1 (poorly cells were treated 5-Fluorouracil (5-Fu), cisplatin, mitomycin c (MMC), FOLFOX (5-Fu, leucovorin, oxaliplatin). To quantify levels or cells, co-stained with annexin V antibodies. percentages single (annexin V-negative, PD-L1-positive; MHC-I-positive) double V-positive, analyzed flow cytometry. Every tested chemotherapeutic agent increased MHC-I, although extents increase differed 5-Fu caused largest increases MHC-I. weakest Notably, chemotherapy-mediated mostly occurred Our findings reveal that mainly

Language: Английский

Citations

0
The role of lipid metabolism in cancer radioresistance DOI

Dandan An,

Danyi Zhai, Chao Wan

et al.

Clinical & Translational Oncology, Journal Year: 2023, Volume and Issue: 25(8), P. 2332 - 2349

Published: April 20, 2023

Language: Английский

Citations

9
Synthesis and evaluation of anti-PD-L1-B11 antibody fragments for PET imaging of PD-L1 in breast cancer and melanoma tumor models DOI Creative Commons
Aditya Bansal, Roxane R. Lavoie, Fabrice Lucien

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 22, 2024

There is a critical need to non-invasively assess the PD-L1 expression in tumors as predictive biomarker for determining efficacy of anti-PD-1/PD-L1 immunotherapies. Non-invasive imaging modality like positron emission tomography (PET) can be powerful tool whole body including multiple metastases patient selection criterion immunotherapy. In this study, we synthesized B11-nanobody, B11-scFv and B11-diabody fragments from full-length anti-PD-L1 B11 IgG. Out three antibody fragments, showed higher nM affinity towards antigen compared B11-nanobody. All were successfully radiolabeled with 64Cu, PET radioisotope. For radiolabeling, first conjugated p-SCN-Bn-NOTA followed by chelation 64Cu. found stable mouse human sera up 24 h. Additionally, all [64Cu]Cu-NOTA-B11-antibody evaluated negative expressing cancer cells subcutaneous tumor models. Based on results, [64Cu]Cu-NOTA-B11-diabody has potential used probe assessing early 4 h post-injection, allowing faster assessment full length IgG based probe.

Language: Английский

Citations

3