CAF-derived miR-642a-3p supports migration, invasion, and EMT of hepatocellular carcinoma cells by targeting SERPINE1 DOI Creative Commons
Shuo Zhang, Gang Cao,

Shuijie Shen

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18428 - e18428

Published: Nov. 11, 2024

Background Cancer-associated fibroblasts (CAFs) and hepatocellular carcinoma (HCC) cells interact to promote HCC progression, but the underlying mechanisms remain unclear. Serpin family E member 1 (SERPINE1) has conflicting roles in HCC, microRNAs (miRNAs) are known regulate tumor progression through intercellular communication. Therefore, we investigated potential involvement of miRNA/SERPINE1 axis crosstalk between CAFs cells. Methods In this study, candidate miRNAs targeting SERPINE1 3′ UTR were predicted using multiple miRNA databases. The mRNA expression Huh7 was assessed after co-culture with RT-qPCR. cell proliferation invasion detected siRNA. functions CAF-derived miR-642a-3p/SERPINE1 examined CCK-8, wound healing, transwell assays, western blot, dual-luciferase reporter assays. Moreover, a orthotopic xenograft model used investigate contribution miR-642a-3p knockdown HCC. Results decreased, while increased co-cultured CAFs. enhanced as well expression. overexpression promoted migration, invasion, epithelial-mesenchymal transition (EMT) by SERPINE1, yielded opposite effect. Rescue experiments confirmed that attenuated inhibitory effects on EMT Importantly, suppressed growth liver tumors. Conclusion facilitated cells, suggesting can be therapeutic target for

Language: Английский

The Role of Small Extracellular Vesicles in Retinoblastoma Development and Progression DOI
Jyothi Attem, Geeta K. Vemuganti

Current Eye Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15

Published: Feb. 4, 2025

A growing body of research on extracellular vesicles (EVs) in cancer has revealed their novel and crucial activities the progression tumors while also paving way for potential therapeutic interventions. It is now known that EVs are natural delivery vehicles particular payloads source cells, enabling them to influence diverse functions cells both healthy malignant cells. In this review, we comprehensively summarize mechanistic insights into sEV roles RB, most frequent intraocular malignancy affects retina young children. We explore sEVs as an emerging area biomarkers targeted therapy. Additionally, address challenges limitations translating sEVs-based technologies clinical practice.

Language: Английский

Citations

0
CAF-derived miR-642a-3p supports migration, invasion, and EMT of hepatocellular carcinoma cells by targeting SERPINE1 DOI Creative Commons
Shuo Zhang, Gang Cao,

Shuijie Shen

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18428 - e18428

Published: Nov. 11, 2024

Background Cancer-associated fibroblasts (CAFs) and hepatocellular carcinoma (HCC) cells interact to promote HCC progression, but the underlying mechanisms remain unclear. Serpin family E member 1 (SERPINE1) has conflicting roles in HCC, microRNAs (miRNAs) are known regulate tumor progression through intercellular communication. Therefore, we investigated potential involvement of miRNA/SERPINE1 axis crosstalk between CAFs cells. Methods In this study, candidate miRNAs targeting SERPINE1 3′ UTR were predicted using multiple miRNA databases. The mRNA expression Huh7 was assessed after co-culture with RT-qPCR. cell proliferation invasion detected siRNA. functions CAF-derived miR-642a-3p/SERPINE1 examined CCK-8, wound healing, transwell assays, western blot, dual-luciferase reporter assays. Moreover, a orthotopic xenograft model used investigate contribution miR-642a-3p knockdown HCC. Results decreased, while increased co-cultured CAFs. enhanced as well expression. overexpression promoted migration, invasion, epithelial-mesenchymal transition (EMT) by SERPINE1, yielded opposite effect. Rescue experiments confirmed that attenuated inhibitory effects on EMT Importantly, suppressed growth liver tumors. Conclusion facilitated cells, suggesting can be therapeutic target for

Language: Английский

Citations

3