Changes in protein phosphorylation by insulin administration in the central nervous system of the gastropod mollusk <i>Lymnaea stagnalis</i> DOI Creative Commons
Junko Nakai, Kengo Namiki, Yuki Totani

et al.

Biophysics and Physicobiology, Journal Year: 2023, Volume and Issue: 20(4), P. n/a - n/a

Published: Jan. 1, 2023

In the gastropod mollusk Lymnaea stagnalis, insulin-like peptides in central nervous system (CNS) control behavioral changes associated with associative learning. Insulin administration to CNS enhances synaptic plasticity involved this type of learning, but it has remained unclear which molecules insulin response cascade are involved. Here, advance a comprehensive analysis, we used two-dimensional electrophoresis and comparative quantitative mass spectrometry perform protein analysis investigating that respond administration. Our results revealed increased phosphorylation AKT RICTOR PI3K/AKT/mTOR signaling cytoskeleton-related proteins. Although was expected were phosphorylated by administration, our findings confirmed correlation between insulin-induced proteins strongly learning memory mechanisms. These contribute elucidate relationship mechanisms not only also various invertebrates vertebrates.

Language: Английский

Impact of fluoxetine exposure on Lymnaea stagnalis and its developing eggs: integrating untargeted lipidomics, targeted metabolomics, and classical risk assessment DOI Creative Commons
Diana Ilyaskina, Yumi Nakadera, M.H. Lamoree

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 4, 2025

Pharmaceuticals such as selective serotonin reuptake inhibitors (SSRIs), are increasingly detected in aquatic environments, posing potential risks to non-target organisms, because many of those substances widely shared neuromodulator. In this study, we investigated the effects SSRI antidepressant, namely, fluoxetine, exposure on freshwater snail L. stagnalis , focusing egg development, neurochemical pathways, and lipid metabolism. Snails were exposed a range 51–434 µg fluoxetine L⁻ 1 for 7 days, followed by analysis survival, feeding behaviour, reproduction, metabolomic changes central nervous system (CNS), albumen gland, eggs. Although no significant observed survival or fecundity, significantly impaired development dose-dependent manner, reducing hatching rates with an EC50 126 . Removal eggs from contaminated environment partially reversed these developmental effects, suggesting recovery if levels decrease. Molecular revealed several lipidomic alterations. CNS, elevated catecholamines, phosphatidylcholines (PC), ceramides linked disruptions neurotransmission, membrane integrity, embryo development. decrease key classes, including sphingomyelins fatty acids, which can be quality. Additionally, histamine both gland suggested further disruption potentially affecting metamorphosis success. Moreover, increase choline, along PC oxidized PC, indicated oxidative stress peroxidation CNS Lymnaea Our findings highlight benefits combining behavioral assessments profiling better understand mechanistic pathways underlying fluoxetine’s adverse effects.

Language: Английский

Citations

0
Genes Upregulated by Operant Conditioning of Escape Behavior in the Pond Snail Lymnaea stagnalis DOI Open Access

Nozomi Chikamoto,

Kanta Fujimoto,

Junko Nakai

et al.

ZOOLOGICAL SCIENCE, Journal Year: 2023, Volume and Issue: 40(5)

Published: July 18, 2023

The pond snail Lymnaea stagnalis is capable of learning by both classical conditioning and operant conditioning. Although related to escape behavior with punishment has been examined some research groups, the molecular mechanisms are not known. In present study, we changes in expression levels cAMP-response element binding protein 1 (CREB1), CREB2, CREB-binding (CBP), monoamine oxidase (MAO) central nervous system (CNS) using real-time PCR following behavior. CREB1 CREB2 transcription factors involved long-term memory Lymnaea; CBP a coactivator CREB1; MAO degrading enzyme for monoamines (e.g., serotonin) important roles Lymnaea. conditioning, cohort, which snails escaping from container encountered aversive KCl, exhibited significantly fewer attempts than control distilled water, during training test periods. After were upregulated, ratio CREB1/CREB2 was also increased, suggesting that involves these factors. content such as serotonin CNS decreased. upregulated genes identified study will help further elucidate

Language: Английский

Citations

4
Sleep-like State in Pond Snails Leads to Enhanced Memory Formation DOI Creative Commons
Kengo Namiki, Junko Nakai, Ken Lukowiak

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(5), P. 336 - 336

Published: May 11, 2024

To test the hypothesis that a sleep-like quiescent state enhances memory consolidation in pond snail Lymnaea stagnalis, we interposed period which snails experienced either quiescent, sleeping or an active, non-sleeping following escape behavior suppression learning (EBSL). During EBSL training, number of escapes made by from container was significantly suppressed using external aversive stimulus (punishment). After were divided into two groups. One group allowed to move freely and experience for 3 h distilled water. The other stimulated with sucrose solution every 10 min keep them active (i.e., non-sleeping). In test, state, whereas not observed kept active. Additionally, latency first shorter than those state. Together, these data are consistent L. stagnalis.

Language: Английский

Citations

1
Changes in protein phosphorylation by insulin administration in the central nervous system of the gastropod mollusk <i>Lymnaea stagnalis</i> DOI Creative Commons
Junko Nakai, Kengo Namiki, Yuki Totani

et al.

Biophysics and Physicobiology, Journal Year: 2023, Volume and Issue: 20(4), P. n/a - n/a

Published: Jan. 1, 2023

In the gastropod mollusk Lymnaea stagnalis, insulin-like peptides in central nervous system (CNS) control behavioral changes associated with associative learning. Insulin administration to CNS enhances synaptic plasticity involved this type of learning, but it has remained unclear which molecules insulin response cascade are involved. Here, advance a comprehensive analysis, we used two-dimensional electrophoresis and comparative quantitative mass spectrometry perform protein analysis investigating that respond administration. Our results revealed increased phosphorylation AKT RICTOR PI3K/AKT/mTOR signaling cytoskeleton-related proteins. Although was expected were phosphorylated by administration, our findings confirmed correlation between insulin-induced proteins strongly learning memory mechanisms. These contribute elucidate relationship mechanisms not only also various invertebrates vertebrates.

Language: Английский

Citations

1