Unlocking the genomic potential of Red Sea coral probiotics

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 28, 2024

The application of beneficial microorganisms for corals (BMC), both in vivo and situ, decreases the bleaching susceptibility mortality rate corals. BMC selection is typically performed via molecular biochemical assays, followed by genomic screening traits. Herein, we present an improved updated silico framework a set six putative strains. We extracted high-quality DNA from coral samples collected Red Sea PacBio sequencing. identified traits mechanisms associated with each strain as well proposed new mechanisms, such chemotaxis presence phages bioactive secondary metabolites. prophages four studied strains suggests their widespread distribution within bacteria. This newly indicates importance BMCs they can expand bacterial metabolic potential offer competitive advantage against pathogens. also detected various metabolites, terpenes, ectoines, lanthipeptides, lasso peptides. These metabolites possess antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant activities play key roles health reducing effects heat stress, high salinity, reactive oxygen species, radiation. Corals are currently facing unprecedented challenges, our revised help select more efficient use studies on microbiome rehabilitation, resilience, restoration.

Language: Английский

Not Only in Corals: Beneficial Microorganisms for Corals can also Be Enriched in Sponges

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Microbiome restoration and rehabilitation using beneficial microorganisms for corals (BMCs) comprise a promising strategy to help cope with anthropogenic stressors. However, there is lack of knowledge on BMC uptake in situ the potential interactions nontarget animals, especially filter-feeders such as sponges. Herein, this study explores whether sponges can acquire BMCs upon direct application these probiotic inoculations affect sponge health. A 4-week experiment applying Stylissa carteri Callyspongia crassa assesses three conditions: no inoculation (control), once thrice week. are naturally available in seawater members native microbiome only S. carteri. The natural presence enriched carteri because their part consortium. Microbiomes both restructured, although not incorporated any C. sample. Both species visually healthy efficiently pumping water at end experiment. These results indicate that nearby unlikely health or performance negatively, even when sponge-native microbiome, suggesting an opportunity development probiotics

Language: Английский

Bacterial inoculation manipulates the coral epigenome

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Summary Environmental shifts can elicit epigenetic modifications in corals, which regulate gene expression and physiology, though it remains unclear if associated bacteria also induce such changes. Here, we inoculated nubbins of the coral Pocillopora verrucosa with an opportunistic pathogen, Vibrio coralliilyticus , and/or a probiotic, Cobetia sp., subjected to heat stress. We discovered that epigenomes responded already after 19 days stress 28 bacterial inoculation. Pathogen led DNA methylation changes not seen other treatments. Notably, probiotic was able mitigate specific glutamate receptor pathway, this correlated increased resilience higher survival rates corals. Importantly, marks persisted even temperature removed, suggesting epigenome acclimation. Thus, bacterial-induced may instigate long-term host resilience.

Language: Английский