Premeiotic endoreplication is the mechanism of obligate parthenogenesis in rock lizards of the genus Darevskia DOI
Dmitrij Dedukh, Marie Altmanová, Ruzanna Petrosyan

et al.

Biology Letters, Journal Year: 2024, Volume and Issue: 20(9)

Published: Sept. 1, 2024

Among vertebrates, obligate parthenogenesis occurs exclusively in squamate reptiles. Premeiotic endoreplication a small subset of developing oocytes has been documented as the mechanism production unreduced eggs minutely explored parthenogenetic lineages, namely teiids and geckos. The situation lacertid genus Darevskia discussed for decades. Certain observations suggested that ploidy level is restored during egg formation through fusion polar body nuclei unisexualis D. armeniaca . In this study, we re-evaluated hypothesis by studying diplotene chromosomes adult females sexual species raddei nairensis parthenogens armeniaca, dahli We revealed 19 bivalents 38 diploid parthenogens, which uncovers premeiotic non-reduced females. earlier contradicting reports can likely be attributed to difficulty identifying mispairing pachytene, fact reptiles relying on only undergo genome doubling overcome pachytene checkpoint. This study highlights co-option escape from reproduction all independent hybrid origins vertebrates studied date.

Language: Английский

Premeiotic endoreplication is the mechanism of obligate parthenogenesis in rock lizards of the genusDarevskia DOI Creative Commons
Dmitrij Dedukh, Marie Altmanová, Ruzanna Petrosyan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 1, 2024

Abstract Among vertebrates, obligate parthenogenesis occurs exclusively in squamate reptiles. Premeiotic endoreplication a small subset of developing oocytes has been documented as the mechanism production unreduced eggs minutely explored parthenogenetic lineages, namely teiids and geckos. The situation lacertid genus Darevskia discussed for decades. Certain observations suggested that ploidy level is restored during egg formation through fusion polar body nuclei D. unisexualis armeniaca . In this study, we re-evaluated hypothesis by studying diplotene chromosomes adult females sexual species raddei nairensis parthenogens armeniaca, dahli We revealed 19 bivalents 38 diploid parthenogens, which uncovers premeiotic non-reduced females. earlier contradicting reports can be likely attributed to difficulty identifying mispairing pachytene, fact reptiles relying on only undergo genome doubling overcome pachytene checkpoint. This study highlights co-option escape from reproduction all independent hybrid origins vertebrates studied date.

Language: Английский

Citations

3
Meiotic deviations and endoreplication lead to diploid oocytes in female hybrids between bighead catfish (Clarias macrocephalus) and North African catfish (Clarias gariepinus) DOI Creative Commons
Dmitrij Dedukh, Artem Lisachov, Thitipong Panthum

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 23, 2024

Introduction Reproductive isolation and hybrid sterility are mechanisms that maintain the genetic integrity of species prevent introgression heterospecific genes. However, crosses closely related can lead to complex evolution, such as formation all-female lineages reproduce clonally. Bighead catfish ( Clarias macrocephalus ) North African C. gariepinus diverged 40 million years ago. They cultivated hybridized in Thailand for human consumption. Male hybrids sterile due genome-wide chromosome asynapsis during meiosis. Although female sometimes fertile, their configuration meiosis has not yet been studied. Methods We analyzed at pachytene (synaptonemal complexes) diplotene (lampbrush chromosomes), using immunostaining detect pairing double-stranded break formation, FISH with species-specific satellite DNAs distinguish parental chromosomes. Results More than 95% oocytes exhibited catfish; however, they were able progress stage form mature eggs. The remaining underwent premeiotic endoreplication, followed by synapsis crossing over between sister chromosomes, similar known clonal fish reptiles. Discussion occurrence reproduction suggests a unique model studying gametogenic alterations caused hybridization potential asexual reproduction. Our results further support view certain animals relies on intrinsic sexually reproducing species, given multiple independent origins same mechanism.

Language: Английский

Citations

1
Premeiotic endoreplication is the mechanism of obligate parthenogenesis in rock lizards of the genus Darevskia DOI
Dmitrij Dedukh, Marie Altmanová, Ruzanna Petrosyan

et al.

Biology Letters, Journal Year: 2024, Volume and Issue: 20(9)

Published: Sept. 1, 2024

Among vertebrates, obligate parthenogenesis occurs exclusively in squamate reptiles. Premeiotic endoreplication a small subset of developing oocytes has been documented as the mechanism production unreduced eggs minutely explored parthenogenetic lineages, namely teiids and geckos. The situation lacertid genus Darevskia discussed for decades. Certain observations suggested that ploidy level is restored during egg formation through fusion polar body nuclei unisexualis D. armeniaca . In this study, we re-evaluated hypothesis by studying diplotene chromosomes adult females sexual species raddei nairensis parthenogens armeniaca, dahli We revealed 19 bivalents 38 diploid parthenogens, which uncovers premeiotic non-reduced females. earlier contradicting reports can likely be attributed to difficulty identifying mispairing pachytene, fact reptiles relying on only undergo genome doubling overcome pachytene checkpoint. This study highlights co-option escape from reproduction all independent hybrid origins vertebrates studied date.

Language: Английский

Citations

0