Lack of membrane sex steroid receptors for mediating rapid endocrine responses in molluscan nervous systems DOI Creative Commons
I Fodor, Shin Matsubara, Tomohiro Osugi

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 12, 2024

Despite the lack of endogenous synthesis and relevant nuclear receptors, several papers have been published over decades claiming that physiology mollusks is affected by natural synthetic sex steroids. With scant evidence for existence functional steroid receptors in mollusks, some scientists speculated effects steroids might be mediated via membrane (i.e. non-genomic/non-classical actions) - a mechanism has well-characterized vertebrates. However, no study yet investigated ligand-binding ability such receptor candidates mollusks. The aim present was to further trace evolution endocrine system investigating presence mollusk, great pond snail ( Lymnaea stagnalis ). We detected sequences homologous known vertebrate transcriptome genome data: G protein-coupled estrogen receptor-1 (GPER1); progestin (mPRs); family C group 6 member A (GPRC6A); Zrt- Irt-like protein 9 (ZIP9). Sequence analyses, including conserved domain analysis, phylogenetics, transmembrane prediction, indicated mPR ZIP9 appeared homologs, while GPER1 GPRC6A seemed non-orthologous receptors. All transiently transfected into HEK293MSR cells were found localized at plasma membrane, confirming they function as signaling assays revealed none interacted with main ligands. Our findings strongly suggest which would ones are not . Although experiments required on other molluscan model species well, we propose both classical non-classical responses specific chordates, systems fundamentally different.

Language: Английский

Lack of membrane sex steroid receptors for mediating rapid endocrine responses in molluscan nervous systems DOI Creative Commons
I Fodor, Shin Matsubara, Tomohiro Osugi

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 12, 2024

Despite the lack of endogenous synthesis and relevant nuclear receptors, several papers have been published over decades claiming that physiology mollusks is affected by natural synthetic sex steroids. With scant evidence for existence functional steroid receptors in mollusks, some scientists speculated effects steroids might be mediated via membrane (i.e. non-genomic/non-classical actions) - a mechanism has well-characterized vertebrates. However, no study yet investigated ligand-binding ability such receptor candidates mollusks. The aim present was to further trace evolution endocrine system investigating presence mollusk, great pond snail ( Lymnaea stagnalis ). We detected sequences homologous known vertebrate transcriptome genome data: G protein-coupled estrogen receptor-1 (GPER1); progestin (mPRs); family C group 6 member A (GPRC6A); Zrt- Irt-like protein 9 (ZIP9). Sequence analyses, including conserved domain analysis, phylogenetics, transmembrane prediction, indicated mPR ZIP9 appeared homologs, while GPER1 GPRC6A seemed non-orthologous receptors. All transiently transfected into HEK293MSR cells were found localized at plasma membrane, confirming they function as signaling assays revealed none interacted with main ligands. Our findings strongly suggest which would ones are not . Although experiments required on other molluscan model species well, we propose both classical non-classical responses specific chordates, systems fundamentally different.

Language: Английский

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