Maintenance of caecal homeostasis by diverse adaptive immune cells in the rhesus macaque DOI Creative Commons
Xaquín Castro Dopico, Mariia V. Guryleva, Marco Mandolesi

et al.

Clinical & Translational Immunology, Journal Year: 2024, Volume and Issue: 13(5)

Published: Jan. 1, 2024

Abstract Objectives The caecum bridges the small and large intestine plays a front‐line role in discriminating gastrointestinal antigens. Although dysregulated acute chronic conditions, tissue is often overlooked immunologically. Methods To address this issue, we applied single‐cell transcriptomic‐V(D)J sequencing to FACS‐isolated CD45 + caecal patch/lamina propria leukocytes from healthy (5‐year‐old) female rhesus macaque ex vivo coupled these data VDJ deep reads haematopoietic tissues. Results We found NK cells ILC3s co‐exist with spectrum of effector T partially derived SOX4 recent thymic emigrants. Tolerogenic Vγ8Vδ1‐T cells, plastic CD4 helper GZMK EOMES TMIGD2 tissue‐resident memory CD8 were present differed metabolically. An IL13 GATA3 Th 2 subset expressing eicosanoid pathway enzymes was accompanied by IL1RL1 regulatory minor proportion IgE plasma (PCs), illustrating tightly regulated type immunity devoid ILC2s. In terms B lymphocyte lineages, patch antigen‐presenting sat alongside germinal centre undergoing somatic hypermutation differentiation into IGF1 PCs. Prototypic gene expression signatures decreased across PC clusters, notably, expanded IgA clonotypes could be traced additional compartments, including bone marrow, supporting that contribute steady stream systemic antibodies. Conclusions advance our understanding immunological function, revealing processes involved barrier maintenance molecular networks relevant disease.

Language: Английский

Maintenance of caecal homeostasis by diverse adaptive immune cells in the rhesus macaque DOI Creative Commons
Xaquín Castro Dopico, Mariia V. Guryleva, Marco Mandolesi

et al.

Clinical & Translational Immunology, Journal Year: 2024, Volume and Issue: 13(5)

Published: Jan. 1, 2024

Abstract Objectives The caecum bridges the small and large intestine plays a front‐line role in discriminating gastrointestinal antigens. Although dysregulated acute chronic conditions, tissue is often overlooked immunologically. Methods To address this issue, we applied single‐cell transcriptomic‐V(D)J sequencing to FACS‐isolated CD45 + caecal patch/lamina propria leukocytes from healthy (5‐year‐old) female rhesus macaque ex vivo coupled these data VDJ deep reads haematopoietic tissues. Results We found NK cells ILC3s co‐exist with spectrum of effector T partially derived SOX4 recent thymic emigrants. Tolerogenic Vγ8Vδ1‐T cells, plastic CD4 helper GZMK EOMES TMIGD2 tissue‐resident memory CD8 were present differed metabolically. An IL13 GATA3 Th 2 subset expressing eicosanoid pathway enzymes was accompanied by IL1RL1 regulatory minor proportion IgE plasma (PCs), illustrating tightly regulated type immunity devoid ILC2s. In terms B lymphocyte lineages, patch antigen‐presenting sat alongside germinal centre undergoing somatic hypermutation differentiation into IGF1 PCs. Prototypic gene expression signatures decreased across PC clusters, notably, expanded IgA clonotypes could be traced additional compartments, including bone marrow, supporting that contribute steady stream systemic antibodies. Conclusions advance our understanding immunological function, revealing processes involved barrier maintenance molecular networks relevant disease.

Language: Английский

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