Transcriptional time-course analysis during ash dieback infection revealed different responses in tolerant and susceptible Fraxinus excelsior genotypes
BMC Plant Biology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 25, 2025
Abstract
Hymenoscyphus
fraxineus
,
the
causal
agent
of
Ash
Dieback
(ADB),
has
been
introduced
to
eastern
Europe
in
1990s
from
where
it
spread
causing
decline
European
ash
populations.
However,
genetic
basis
molecular
response
tolerant
and
susceptible
trees
this
disease
is
still
largely
unknown.
We
performed
RNA-sequencing
study
transcriptomic
four
genotypes
(ADB-tolerant
FAR3
FS36,
ADB-susceptible
UW1
UW2),
during
a
time-course
7,
14,
21,
28
days
post-inoculation,
including
mock-inoculated
as
control
samples
for
each
sampling
time
point.
The
analysis
yielded
395
500
Differentially
Expressed
Genes
(DEGs)
along
ADB-tolerant
respectively,
while
UW2
revealed
194
571
DEGs,
with
most
DEGs
found
exclusively
just
one
genotypes.
shared
between
included
genes
involved
production
phytoalexins
other
secondary
metabolites
roles
plant
defense.
Moreover,
we
identified
an
earlier
expression
both
pattern-
effector-triggered
immunity
(PTI
ETI)
genotypes,
responses
were
delayed
(late
response).
Overall,
these
results
different
patterns
not
only
but
also
within
two
groups.
This
hints
individual
natural
tolerance
ADB,
possibly
revealing
diversified
strategies
across
Language: Английский
Transcriptional profiling of Fraxinus excelsior leaves during the early infection phase of ash dieback
Journal of Plant Diseases and Protection,
Journal Year:
2024,
Volume and Issue:
132(1)
Published: Dec. 21, 2024
Abstract
Ash
dieback
(ADB)
has
been
causing
the
progressive
decline
of
Fraxinus
excelsior
trees
throughout
Europe,
urging
research
and
forest
management
to
develop
strategies
combat
ADB.
A
genetically
heritable
component
in
susceptibility
this
fungal
disease
was
reported
common
gardens.
Thus,
exploring
molecular
basis
ADB
will
further
support
breeding
initiatives
future.
We
performed
transcriptional
profiling
infected
uninfected
leaves
from
two
ash
genotypes
with
different
Hymenoscyphus
fraxineus
.
Leaf
rachises
were
sampled
one
week
following
inoculation.
Differential
gene
expression
analysis
compare
between
treatments
each
genotype
(individual
response)
or
combined
(common
response).
Due
heterogeneity
response,
only
DEGs
discussed
that
passed
stringent
assessment.
Our
results
revealed
UW1,
most
susceptible
genotype,
showed
a
total
515
differentially
expressed
genes
(DEGs),
some
them
possibly
suggesting
self-control
mechanism,
hindering
an
effective
immune
response
increased
susceptibility.
On
other
hand,
FAR3,
least
230
DEGs,
seemed
induce
contained
but
more
efficient
hinting
toward
salicylic
acid-mediated
process
activating
pathogen-related
(like)
proteins
as
thaumatin-like,
peroxidases,
chitinases.
In
512
modulated
transcripts
phenylpropanoid
pathway
commonly
altered
both
genotypes.
Altogether,
work
comprised
initial
exploration
including
selected
distinct
ADB,
however,
heterogenous
indicated
need
improve
experimental
inoculation
approach.
Exploring
patterns
holds
promise
reveal
early
mechanisms,
new
markers
related
susceptibility,
well
contribute
developing
may
help
contain
Language: Английский
Eschentriebsterben eindämmen durch Mikrobiom-Optimierung?
BIOspektrum,
Journal Year:
2024,
Volume and Issue:
30(6), P. 634 - 636
Published: Oct. 1, 2024
Abstract
‘FraxForFuture’
was
an
interdisciplinary
project
to
study
the
devastating
effects
of
ash
dieback
on
host,
characterize
associated
microbiome,
and
develop
non-chemical
biological
control
strategies
combat
pathogen,
Hymenoscyphus
fraxineus
.
Bacteria
fungi
as
well
fungal
secondary
metabolites
were
identified,
which
have
antagonistic
effect
against
pathogen
or
can
be
used
for
microbiome
optimisation.
Results,
conclusions,
outlook
are
presented.
Language: Английский