The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 2, 2024

Abstract The adaptive immune response during BCoV infection of peripheral blood mononuclear cells (PBMCs), the bovine spleen cells, and their isolated T lymphocytes was not studied well. Our study confirmed successful in PBMCs cells. This evidenced by measuring genome copy numbers using real-time PCR, expression levels spike nucleocapsid proteins via western blot immunofluorescence assays, virus infectivity titration plaque assay. In infected PBMCs, CD4 T-cell were 1.45-fold higher, CD8 1.6-fold lower compared to sham-infected Conversely, splenocytes showed a 0.88-fold decrease T-cells 1.88-fold increase T-cells. cytokine gene analysis revealed that activated type 1 interferon upregulated IL-6 splenocytes. These findings demonstrate successfully infects from spleen, inducing differential host favors replication.

Language: Английский

The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication

Virology, Journal Year: 2024, Volume and Issue: 600, P. 110259 - 110259

Published: Oct. 18, 2024

Language: Английский