Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 21, 2025

Abstract Enzyme deployment is proliferating extensively in industries owing to their environmentally friendly and easily degradable attributes. This article undertakes an exhaustive examination of wild subtilisin enzyme, covering purification, biochemical delineation, analytical techniques, practical implementations. The purification methodology involved partial refinement, anionic exchange, gel filtration chromatography, culminating a factor 3.406, corroborated by SDS-PAGE showcasing molecular weight ~ 42 kDa. Biochemical scrutiny unveiled the enzyme's response, with optimal pH at 9 temperature peak 60 ℃. Various surfactants, metal ions, organic solvents inhibitors exhibited notable efficacy. Substrate specificity kinetics showcased utmost N-Suc-F-A-A-F- p NA, registering K m V max values 0.731 ± 0.5 mM 0.87 × 10 3 U/mg, respectively. Different bioanalytical techniquesproffered insights into structural biophysical facets. Practical applications encompassed goat skin depilation, feather disintegration, blood clot dissolution, exemplifying multifaceted utility. To embark upon elucidation structure–function relationships, three-dimensional model was devised through homology modelling, leveraging existing structures (PDB: 3WHI). Molecular docking score − 8.8 kcal/mol dynamic simulations augmented comprehension interactions NA. research significantly contributes unravelling intricacies underscores potential industrial biomedical prowess. Subtilisin can be explored for its thrombolytic several cardiovascular diseases. It may aid management thrombosis dissolving clots conditions like deep pulmonary embolism, myocardial infarction, ischemic strokes, atherosclerosis breaking down fibrin arterial plaques, thus preventing heart attacks strokes. Graphical

Language: Английский