Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression DOI
Anna-Lee C Thompson,

Judith Wopereis,

Yonas I. Tekle

et al.

Microscopy and Microanalysis, Journal Year: 2025, Volume and Issue: 31(2)

Published: March 17, 2025

Microscopy approaches are frequently used to decipher the localization and quantify abundance of biologically relevant molecular targets within single cells. Recent research has applied many optical imaging techniques specifically visualize epigenetic modifications, mechanisms by which organisms control gene expression in response environmental factors. While omics-based understand mechanisms, provide spatial information that supplies greater context for discerning function. Thus, labeling have been developed using various fluorescence microscopy, electron super-resolution microscopy techniques. Here, we synthesize about methods enable visualization marks including DNA methylation, histone RNAs, insights into involved chromatin remodeling expression. The ability determine how where specific manifest structurally functionally cells demonstrates power aiding our understanding processes.

Language: Английский

Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression DOI
Anna-Lee C Thompson,

Judith Wopereis,

Yonas I. Tekle

et al.

Microscopy and Microanalysis, Journal Year: 2025, Volume and Issue: 31(2)

Published: March 17, 2025

Microscopy approaches are frequently used to decipher the localization and quantify abundance of biologically relevant molecular targets within single cells. Recent research has applied many optical imaging techniques specifically visualize epigenetic modifications, mechanisms by which organisms control gene expression in response environmental factors. While omics-based understand mechanisms, provide spatial information that supplies greater context for discerning function. Thus, labeling have been developed using various fluorescence microscopy, electron super-resolution microscopy techniques. Here, we synthesize about methods enable visualization marks including DNA methylation, histone RNAs, insights into involved chromatin remodeling expression. The ability determine how where specific manifest structurally functionally cells demonstrates power aiding our understanding processes.

Language: Английский

Citations

0