Investigating the Transformation Products of Selected Antibiotics and 17 α-Ethinylestradiol under Three In Vitro Biotransformation Models for Anticipating Their Relevance in Bioaugmented Constructed Wetlands DOI Creative Commons

Lucas Sosa Alderete,

Andrés Sauvêtre, Serge Chirón

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(6), P. 508 - 508

Published: June 5, 2023

The degradation of three antibiotics (sulfamethoxazole, trimethoprim, and ofloxacin) one synthetic hormone (17 α-ethinylestradiol) was investigated in in-vitro biotransformation models (i.e., pure enzymes, hairy root, Trichoderma asperellum cultures) for anticipating the relevance formation transformation products (TPs) constructed wetlands (CWs) bioaugmented with T. fungus. identification TPs carried out employing high-resolution mass spectrometry, using databases, or by interpreting MS/MS spectra. An enzymatic reaction β-glucosidase also used to confirm presence glycosyl-conjugates. results showed synergies mechanisms between these models. Phase II conjugation reactions overall glycosylation predominated root cultures, while phase I metabolization (e.g., hydroxylation N-dealkylation) cultures. Following their accumulation/degradation kinetic profiles helped determining most relevant TPs. Identified contributed residual antimicrobial activity because metabolites can be more reactive glucose-conjugated transformed back into parent compounds. Similar other biological treatments, CWs is concern deserves simple vitro avoid complexity field-scale studies. This paper brings new findings on emerging pollutants metabolic pathways established model plants, including extracellular enzymes.

Language: Английский

Investigating the Transformation Products of Selected Antibiotics and 17 α-Ethinylestradiol under Three In Vitro Biotransformation Models for Anticipating Their Relevance in Bioaugmented Constructed Wetlands DOI Creative Commons

Lucas Sosa Alderete,

Andrés Sauvêtre, Serge Chirón

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(6), P. 508 - 508

Published: June 5, 2023

The degradation of three antibiotics (sulfamethoxazole, trimethoprim, and ofloxacin) one synthetic hormone (17 α-ethinylestradiol) was investigated in in-vitro biotransformation models (i.e., pure enzymes, hairy root, Trichoderma asperellum cultures) for anticipating the relevance formation transformation products (TPs) constructed wetlands (CWs) bioaugmented with T. fungus. identification TPs carried out employing high-resolution mass spectrometry, using databases, or by interpreting MS/MS spectra. An enzymatic reaction β-glucosidase also used to confirm presence glycosyl-conjugates. results showed synergies mechanisms between these models. Phase II conjugation reactions overall glycosylation predominated root cultures, while phase I metabolization (e.g., hydroxylation N-dealkylation) cultures. Following their accumulation/degradation kinetic profiles helped determining most relevant TPs. Identified contributed residual antimicrobial activity because metabolites can be more reactive glucose-conjugated transformed back into parent compounds. Similar other biological treatments, CWs is concern deserves simple vitro avoid complexity field-scale studies. This paper brings new findings on emerging pollutants metabolic pathways established model plants, including extracellular enzymes.

Language: Английский

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