Characterization and Analysis of the Functional Differences of the Two Eclosion Hormones in Regulating Molting in the White Shrimp Litopenaeus vannamei

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12813 - 12813

Published: Nov. 28, 2024

Litopenaeus vannamei, with an annual production of 5–6 million tons and a value USD 50–60 billion, is cornerstone global aquaculture. However, molting-related losses 5–20% significantly impact this industry, the physiological mechanisms molting remain unclear. This study aims to elucidate role eclosion hormone (EH) in regulation enhances understanding physiology L. vannamei. investigated vannamei regulation. Two EH cDNAs, LvEH I II, were identified, their expression patterns across tissues seven stages (A, B, C, D0, D1, D2, D3) analyzed. was predominantly expressed gill, epidermis, eyestalk, while II mainly eyestalk brain. highly gills at D2 D3 molting, whereas both (brain) (eyestalk) stages. RNA interference (RNAi) targeting revealed its critical as silencing disrupted molting-regulation genes, ETH, CCAP, CHH, CDA, bursicon (Burs), delaying process. These findings highlight indispensable for normal provide foundation developing effective management strategies reduce industry losses.

Language: Английский

Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, Scylla paramamosain

Animals, Journal Year: 2024, Volume and Issue: 14(24), P. 3706 - 3706

Published: Dec. 23, 2024

In this study, we identified Sp-ACP and its putative receptor from the mud crab S. paramamosain explored potential role in ovarian development. RT-PCR results suggested was extensively expressed nervous tissues, ovary, middle gut, Y-organ, while Sp-ACPR highly ovary. The expression level of eyestalk, cerebral ganglia gradually increased during development, whereas exhibited an opposite pattern Immunofluorescence revealed that ACP specifically localized follicle cells vitro experiments showed vitellogenin (Sp-VgR) ovary significantly by 4 6 h incubation (10 nM). addition, 12 injection induced levels Sp-Vg hepatopancreas Sp-VgR hemolymph 17β-estradiol titer. Finally, it demonstrated prolonged Vg VgR expression, titer, GSI, oocyte diameter. conclusion, our is involved regulation development paramamosain, likely inducing through estradiol promoting uptake oocytes.

Language: Английский